Acetoanaerobium sticklandii DSM 519 is a hyperammonia‐producing anaerobe that catabolizes proteins and amino acids into organic solvents and volatile acids via the Stickland reactions. However, the specific growth rate and metabolic capability of this organism on proteins and amino acids are not yet known. Therefore, the present study was intended to evaluate its specific growth rate and metabolic potential on gelatin and amino acids in the experimental media. We carried out metabolic assay experiments to calculate its ability to utilize pure gelatin, single amino acids, and amino acid pairs at different growth phases. The results of this study show that complete assimilation of gelatin was achieved by its log‐phase culture. The subsequent fermentation of amino acids was much faster than gelatin hydrolysis. The rate of gelatin degradation was associated with the growth and catabolic rates of this organism. Many amino acids were not assimilated completely for its growth and energy conservation. A log‐phase culture of this organism preferably utilized l‐cysteine, l‐arginine, and l‐serine, and released more fraction of ammonia. As shown by our analysis, the catabolic rates of these amino acids were determined by the rates of respective enzymes involved in amino acid catabolic pathways and feedback repression of ammonia. The growth kinetic data indicated that at the initial growth stage, a metabolic shift in its solventogenesis and acidogenesis phases was associated with catabolism of certain amino acids. Thus, the results of this study provide a new insight to exploit its log‐phase culture as a starter for the production of biofuel components from gelatin processing industries.

中文翻译：

---

Acetoanaerobiumsticklandii DSM 519 对明胶和氨基酸的生长相关分解代谢潜力

Acetoanaerobiumsticklandii DSM 519 是一种产生高氨的厌氧菌，通过 Stickland 反应将蛋白质和氨基酸分解为有机溶剂和挥发性酸。然而，这种生物对蛋白质和氨基酸的特定生长速率和代谢能力尚不清楚。因此，本研究旨在评估其在实验培养基中对明胶和氨基酸的特定生长速率和代谢潜力。我们进行了代谢测定实验，以计算其在不同生长阶段利用纯明胶、单一氨基酸和氨基酸对的能力。这项研究的结果表明，明胶的完全同化是通过其对数期培养实现的。随后氨基酸的发酵比明胶水解快得多。明胶降解率与该生物的生长和分解代谢率有关。许多氨基酸由于其生长和能量保存而未被完全同化。这种生物的对数期培养优选使用 l-半胱氨酸、l-精氨酸和 l-丝氨酸，并释放更多的氨。正如我们的分析所示，这些氨基酸的分解代谢率由参与氨基酸分解代谢途径和氨的反馈抑制的各个酶的速率决定。生长动力学数据表明，在初始生长阶段，其溶剂生成和酸生成阶段的代谢转变与某些氨基酸的分解代谢有关。因此，