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Surface plasmon resonance signal enhancement based on erlotinib loaded magnetic nanoparticles for evaluation of its interaction with human lung cancer cells
Optics & Laser Technology ( IF 5 ) Pub Date : 2020-08-18 , DOI: 10.1016/j.optlastec.2020.106521
Saeideh Mohammadzadeh-Asl , Ayuob Aghanejad , Miguel de la Guardia , Jafar Ezzati Nazhad Dolatabadi , Ahmad Keshtkar

Surface plasmon resonance (SPR) sensor provides a very useful tool based on its label-free, real-time monitoring and low price properties. However, measurement of small molecules and extremely diluted analytes is difficult and therefore, signal enhancement is required. In the present study, signal enhancement of erlotinib conjugated magnetic nanoparticles (erlotinib-MNPs) compared to erlotinib was evaluated via their interaction with overexpressed epidermal growth factor receptor on human lung cancer cells (A549 cell line) surface using SPR sensor at three temperature levels. The attained results showed an average signal amplification of about 2.5-fold for MNP-erlotinib interaction with A549 cells compared to erlotinib interaction with those cells. Overall, this signal enhancement method could be used for measurement of small molecules and cancer cell detection via interaction of their overexpressed receptor and various ligands.



中文翻译:

基于负载厄洛替尼的磁性纳米粒子的表面等离子体共振信号增强,用于评估其与人肺癌细胞的相互作用

表面等离子体共振(SPR)传感器基于其无标签,实时监控和低价格特性,提供了非常有用的工具。但是,难以测量小分子和极度稀释的分析物,因此需要增强信号。在本研究中,通过在三个温度水平下使用SPR传感器,通过与人肺癌细胞(A549细胞系)表面上过表达的表皮生长因子受体的相互作用,评估了与厄洛替尼相比的厄洛替尼共轭磁性纳米颗粒(erlotinib-MNPs)的信号增强。获得的结果表明,与埃洛替尼与那些细胞的相互作用相比,MNP-埃洛替尼与A549细胞的相互作用的平均信号放大率约为2.5倍。总体,

更新日期:2020-08-18
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