A ‘heart-cut’ multidimensional gas chromatography‒mass spectrometry (H/C MDGC‒MS) method for separation and identification of triacylglycerols (TAGs) in extra virgin olive oil was developed. A GC configuration, comprising a non-polar first dimension (¹D) column (15 m length) and a mid-polarity second dimension (²D) column (9 m length), was employed. Standard TAGs were used to test and demonstrate the H/C MDGC method, for identification of TAG components and to validate the method. Various chromatographic conditions such as column flow and temperature program were evaluated. The ¹D separation resulted in overlap of some standard TAG peaks. These overlapped ¹D regions of the standard TAGs were H/C to ²D for further separation and resulted in clearly distinguished individual TAG component peaks. The ¹D separation of olive oil TAGs displayed three major peaks and four minor peaks. The application of the H/C MDGC method to olive oil TAGs resulted in the separation of each sampled ¹D region into two or more TAG peaks. TAG components in olive oil resolved on the ²D column were identified based on characteristic mass fragment ions such as [M–RCO₂]⁺, [RCO+128]⁺, [RCO+74]⁺ and RCO⁺ and comparison of their mass spectra with that of the standard TAGs. Sixteen olive oil TAGs were identified by MS after ²D separation. The repeatability of the H/C method was evaluated in terms of retention time shift and area response in the ²D and found to be <0.02% and <8% RSD respectively.

开发了一种“心切”多维气相色谱-质谱法（H / C MDGC‒MS），用于分离和鉴定特级初榨橄榄油中的三酰基甘油（TAGs）。采用了一种GC配置，包括一个非极性的第一维（¹ D）色谱柱（15 m长）和一个中等极性的第二维（² D）色谱柱（9 m长）。标准TAG用于测试和演示H / C MDGC方法，用于识别TAG组件并验证该方法。评估了各种色谱条件，例如柱流量和温度程序。的¹ d分离导致的一些标准TAG峰重叠。这些重叠的^1个标准TAG的d区分别为H / C至²D进行进一步分离，并得到清晰区分的单个TAG组分峰。的¹橄榄油TAG的分离d显示三个主要峰和四个次要峰。H / C MDGC方法对橄榄油TAG的应用导致将每个采样的¹ D区分离为两个或多个TAG峰。在解决在橄榄油TAG部件² d柱中鉴定基于特征质量碎片离子，如[M-RCO ₂ ] ⁺，[RCO + 128] ⁺，[RCO + 74] ⁺和RCO ⁺和它们的质量的比较具有标准TAG的光谱。^2次后MS鉴定出16个橄榄油TAGD分离。的H / C方法的重复性在保留时间漂移和面积响应来评价² d，发现为<分别为0.02％和<8％RSD。