Efficient and economical separation and enrichment of high-content class compounds from complex natural plants are of great importance. This study describes a novel continuous chromatography system (CCS) with multi-zone and multi-column dynamic tandem techniques for the efficient and economical separation and enrichment of high-content class compounds from natural products. The CCS was split into eight zones (each zone covered 2 or 3 columns connected dynamically in a series) via a multi-channel logic control valve to continuously and automatically separate and enrich class compounds from complex natural plants. CCS separation conditions were optimized by static and dynamic adsorption and desorption experiments. With the CCS system, 120.82 kg of Panax notoginseng saponins (PNS) was isolated from 1.0 t of P. notoginseng. Importantly, the total contents of five main saponins (R₁, Rg₁, Re, Rb₁, and Rd) in PNS exceeded 90%, and the recovery rate exceeded 85%. In addition, this separation system enhanced the separation efficiency and decreased the mobile phase used. Thus, CCS with multi-zone and multi-column dynamic tandem techniques is an efficient and economical method for the large-scale preparation of high-content total saponins from P. notoginseng and will have wide application prospects, especially in the large-scale preparation of high-content class compounds from natural plants.

从复杂的天然植物中高效，经济地分离和富集高含量的化合物非常重要。这项研究描述了一种新颖的连续色谱系统（CCS），该系统具有多区域和多列动态串联技术，可从天然产物中高效经济地分离和富集高含量的化合物。CCS通过多通道逻辑控制阀分为八个区域（每个区域覆盖2或3列动态串联的区域），以连续自动地从复杂的天然植物中分离和富集分类化合物。通过静态和动态吸附与解吸实验优化了CCS分离条件。通过CCS系统，从1.0吨的三七中分离出120.82公斤三七总皂苷（PNS）。三七。重要的是，PNS中五种主要皂苷（R ₁，Rg ₁，Re，Rb ₁和Rd）的总含量超过90％，回收率超过85％。另外，该分离系统提高了分离效率并减少了使用的流动相。因此，采用多区多柱动态串联技术的CCS是一种高效，经济的大规模生产三七总皂苷的方法，特别是在大规模制备中具有广阔的应用前景。天然植物中高含量的化合物。