当前位置: X-MOL 学术Cryobiology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Morphological abnormalities in zebrafish cryopreserved sperm
Cryobiology ( IF 2.7 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.cryobiol.2020.08.003
Bruna Bitencourt da Costa 1 , Diogo Losh de Oliveira 2 , Rômulo Batista Rodrigues 1 , Itamar Cossina Gomes 1 , Danilo Pedro Streit 1
Affiliation  

The aim of the present study was to evaluate the effect of cryopreservation on the morphology of zebrafish sperm (Danio rerio). Sperm from 30 males were collected and divided in two treatments: fresh and cryopreserved semen. The following were measured sperm morphology, motility and membrane integrity. Cryopreservation reduced motility, the number of normal cells and the membrane integrity, as well as increased the percentage of sperm abnormalities. The most frequent types of morphological changes found in cryopreserved semen were macrocephaly, loose head, degenerated head, proximal gout, curled tail and short tail. This study opens the way for further investigations on morphological changes and for a new classification of these changes in fish semen due to cryopreservation.

中文翻译:

斑马鱼冷冻保存精子的形态异常

本研究的目的是评估冷冻保存对斑马鱼精子(Danio rerio)形态的影响。收集了 30 名男性的精子并将其分为两种处理方式:新鲜精液和冷冻保存的精液。以下分别测量精子形态、运动性和膜完整性。冷冻保存降低了运动性、正常细胞的数量和膜的完整性,并增加了精子异常的百分比。在冷冻保存的精液中发现的最常见的形态变化类型是大头畸形、头部松散、头部退化、近端痛风、卷尾和短尾。这项研究为进一步研究形态变化和对冷冻保存引起的鱼类精液变化的新分类开辟了道路。
更新日期:2020-12-01
down
wechat
bug