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Recent developments in the use of peroxygenases - Exploring their high potential in selective oxyfunctionalisations.
Biotechnology Advances ( IF 16.0 ) Pub Date : 2020-08-19 , DOI: 10.1016/j.biotechadv.2020.107615
Markus Hobisch 1 , Dirk Holtmann 2 , Patricia Gomez de Santos 3 , Miguel Alcalde 4 , Frank Hollmann 5 , Selin Kara 1
Affiliation  

Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.



中文翻译:

过氧化酶使用的最新进展——探索它们在选择性氧化功能化方面的巨大潜力。

过氧化酶是一类新兴的酶,允许以与众所周知的 P450 单加氧酶不同的辅助因子独立方式进行选择性氧化功能化反应。在此,我们重点关注有机合成、分子生物技术和反应工程方面的最新进展,这些观点致力于将这些酶应用于工业应用。这涵盖了来自不同来源的自然多样性、用于表达的蛋白质工程策略、底物范围、活性和选择性、通过固定化酶的稳定性以及过氧化酶在低水介质中的使用。我们相信过氧化酶可以为选择性氧化功能化提供很多东西,我们有很多东西需要研究来探索这些多功能生物催化剂在有机合成中的全部潜力。

更新日期:2020-08-19
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