Cleft lip and palate are the most common congenital abnormalities that occur early in pregnancy. The majority of cranial mesenchyme is derived from cranial neural crest cells that differentiate into odontoblasts, cartilage, craniofacial bone, and connective tissue. A subset of these cells differentiates into cranial ganglia. We have previously reported an induction protocol of cranial neural crest cell-like cells from human pluripotent stem cells. This study tested detection of the cytotoxic sensitivities of dental materials, including titanium ions, palladium ions, and hydroxyethyl methacrylate, on the cell viability of induced cranial neural crest cell-like cells (iNC-LCs) derived from Tic human induced pluripotent stem cell (hiPSC) line. Further, the sensitivity was compared with those of human fetal lung fibroblastic cell line MRC-5, which is origin of Tic hiPSC, and osteoblastic cell line MC3T3-E1 which was derived from mouse calvaria. The results suggested that this cell-based assay system using iNC-LCs is a potential method for in vitro screening as an alternative to animal testing to predict toxic effects of dental materials on early craniofacial development.

唇left裂是妊娠早期最常见的先天性异常。大部分颅间质来自颅神经c细胞，它们分化为成牙本质细胞，软骨，颅面骨和结缔组织。这些细胞的一部分分化为颅神经节。先前我们已经报道了来自人多能干细胞的颅神经-细胞样细胞的诱导方案。这项研究测试了牙科材料（包括钛离子，钯离子和甲基丙烯酸羟乙酯）对由Tic人诱导的多能干细胞（iNC-LC）诱导的颅神经rest细胞样细胞（iNC-LC）的细胞活力的细胞毒性敏感性检测。 hiPSC）行。此外，将敏感性与人类胎儿肺成纤维细胞系MRC-5的敏感性进行了比较，它是Tic hiPSC的起源，而成骨细胞系MC3T3-E1来源于小鼠颅盖。结果表明，这种使用iNC-LC的基于细胞的测定系统是一种潜在的体外筛选方法，可以替代动物测试来预测牙科材料对早期颅面发育的毒性作用。