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Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2020-07-20 , DOI: 10.1007/s10658-020-02058-x
Juan M. Arjona-López , Paul Telengech , Nobuhiro Suzuki , Carlos J. López-Herrera

Many Mediteranean isolates of Rosellinia necatrix, causing root rot in avocado, have previously been screened for viruses. Among them is isolate Rn459 that has been shown to be infected by at least three viruses such as Rosellinia necatrix partitivirus 10 (RnPV10), Rosellinia necatrix hypovirus 2 (RnHV2), Rosellinia necatrix fusagravirus 1 (RnFGV1), and Rosellinia necatrix virga-like virus (RnVLV). Here, we attempted to eliminate the viruses by hyphal tip cultures to examine their effect on colony growth in vitro and virulence on avocado plants. The obtained fungal strain termed, Rn459_PV10F/VLVF, which was confirmed to be cured of RnPV10 and RnVLV, but still retaining RnHV2, manifested a phenotype different from the original Rn459. Colony growth comparison showed that Rn459_PV10F/VLVF grew faster than the original Rn459 isolate and the virulence on avocado plants of this Rn459_PV10F/VLVF strain was higher than the original Rn459 strain. These combined results suggest that RnPV10 and RnVLV, alone or together, contribute to confer hypovirulence on the R. necatrix isolates.



中文翻译:

Partitivirus和virga-like病毒共同感染Rosellinia necatrix与低毒力有关

玫瑰茄的许多地中海分离株曾在牛油果中引起根腐病,以前已经过筛查病毒。其中有分离株Rn459,已证明已被至少三种病毒感染,如中性玫瑰茄10(RnPV10),中性玫瑰茄次流感病毒2(RnHV2),中性玫瑰茄镰刀形病毒1(RnFGV1)和类似中性玫瑰茄病毒的病毒。 (RnVLV)。在这里,我们试图通过菌丝尖端培养消除病毒,以检查它们对体外菌落生长和鳄梨植物毒力的影响。获得的命名为Rn459_PV10F / VLVF的真菌菌株已被证实可治愈RnPV10和RnVLV,但仍保留RnHV2,表现出与原始Rn459不同的表型。菌落生长比较显示,Rn459_PV10F / VLVF的生长速度快于原始Rn459分离株,该Rn459_PV10F / VLVF菌株在鳄梨植物上的毒力高于原始Rn459菌株。这些综合结果表明,RnPV10和RnVLV单独或一起有助于赋予猪低毒力。R. necatrix分离株。

更新日期:2020-08-19
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