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Comparative analysis of morphological and molecular approaches integrated into the study of the dinoflagellate biodiversity within the recently deposited Black Sea sediments - benefits and drawbacks.
Biodiversity Data Journal ( IF 1.3 ) Pub Date : 2020-08-18 , DOI: 10.3897/bdj.8.e55172
Nina Dzhembekova 1 , Fernando Rubino 2 , Satoshi Nagai 3 , Ivelina Zlateva 1 , Nataliya Slabakova 1 , Petya Ivanova 1 , Violeta Slabakova 4 , Snejana Moncheva 1
Affiliation  

One of the assets, assigned to the phytoplankton resting stages, is that of serving as the “memory” of the aquatic ecosystems and preserved biodiversity in the course of time. However, an accurate cyst identification proves to be a more difficult and extremely challenging process, even today. In order to gain a better taxonomic coverage of cyst assemblages in the Black Sea, an integrated approach of the classical morphological identification with metabarcoding methods (MySeq sequencing of V7-V9 regions of the 18S rDNA) was applied on thirteen surface sediment samples collected from different sites. A total number of 112 dinoflagellate taxa was detected at the species level and ascribed to 51 genera. In general, it is the molecular analysis that yields a higher number of taxa as compared to those obtained through the morphological taxonomy (66 taxa based on the DNA sequences versus 56 morphologically-identified taxa). Besides, it should be pointed out that the integrated dataset includes 14 potentially toxic dinoflagellate species. Discerned, subsequently, was a good dataset consistency for ten species, followed by some discrepancies as to a number of taxa, identified with one of the methods only, due to specific methodological biases. On the whole, it could be concluded that the combination of morphological and molecular methods is likely to increase the potential for a more reliable taxonomic assessment of phytoplankton diversity in marine sediments which, in turn, proves conclusively the utmost importance of the integrated approach.

中文翻译:

形态和分子方法的比较分析已整合到最近沉积的黑海沉积物中对鞭毛生物多样性的研究中-利弊。

分配给浮游植物休息阶段的资产之一是随着时间的流逝而成为水生生态系统和保存的生物多样性的“记忆”。然而,即使在今天,准确的囊肿识别也被证明是一个更加困难和极具挑战性的过程。为了在黑海中获得更好的囊肿组合分类学覆盖率,将经典的形态学识别与元条形码方法(18S rDNA的V7-V9区的MySeq测序)进行整合的方法应用于从不同地方收集的13个表面沉积物样品网站。在物种水平上检测到总数为112个鞭毛类,归因于51属。一般来说,与通过形态分类法获得的分类相比,分子分析产生的分类单元数量更高(基于DNA序列的分类单元为66个,而形态学鉴定的分类单元为56个)。此外,应该指出的是,综合数据集包括14种潜在的有毒鞭毛藻物种。随后发现,十个物种具有良好的数据集一致性,随后由于特定方法的偏见,仅在其中一种方法中识别出一些分类单元,就出现了一些差异。总体而言,可以得出结论,形态学和分子方法的结合可能会增加对海洋沉积物中浮游植物多样性进行更可靠的分类学评估的潜力,这反过来最终证明了综合方法的最重要意义。
更新日期:2020-08-18
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