Nonalcoholic fatty liver disease (NAFLD) is one of the most common causes of chronic liver disease worldwide. Current studies have shown that PNPLA3 (Patatin-like phospholipase domain containing 3) rs738409 G/C gene polymorphism is associated with adult nonalcoholic fatty liver disease [1, 2].But there is no consensus on the relationship between PNPLA3 rs738409 G/C gene polymorphism and children NAFLD due to differences in population samples. To this end, a meta-analysis of published research is conducted to comprehensively assess the relationship between PNPLA3 gene polymorphism and NAFLD in children. We searched MEDLINE, PubMed, EMBASE, and CENTRAL databases from inception to May 2019. Case-control studies assessing the relationship between PNPLA3 rs738409 G/C gene polymorphism with non-alcoholic fatty liver disease in children were selected according to inclusion and exclusion criteria. Random effects model was used to quantify the association between the PNPLA3 rs738409 G/C gene polymorphism and the susceptibility of children’s NAFLD. Fixed effects model was used to quantify the relationship between the PNPLA3 rs738409 G/C gene polymorphism and the severity of NAFLD in children. The Stata 12.0 software was employed for data analysis. A total of nine case-control studies were included in this meta-analysis containing data of 1173 children with NAFLD and 1792 healthy controls. Five studies compared NAFLD children and non-NAFLD healthy populations. Statistical analysis showed that PNPLA3 gene polymorphism was significantly associated with children’s NAFLD in the allele contrast, dominant, recessive and over dominant models (G vs C,OR = 3.343, 95% CI = 1.524–7.334; GG + GC vs CC,OR = 3.157, 95% CI = 1.446–6.892;GG vs GC + CC,OR = 5.692, 95% CI = 1.941–16.689; GG + CC vs GC,OR = 2.756, 95% CI = 1.729–4.392). Four case-control studies compared Children with nonalcoholic fatty liver (NAFL) and children with nonalcoholic steatohepatitis (NASH). The results showed that the PNPLA3 gene polymorphism was also significantly associated with the severity of NAFLD in children in recessive gene model (GG vs GC + CC,OR = 14.43, 95% CI = 5.985–34.997); The Egger’s test revealed no significant publication bias. Meta-analysis showed that PNPLA3 gene polymorphism was significantly associated with susceptibility and severity of NAFLD in children.

中文翻译：

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PNPLA3 rs738409 G / C基因多态性与儿童非酒精性脂肪性肝病的关联：一项荟萃分析。

非酒精性脂肪肝疾病（NAFLD）是全球慢性肝病的最常见原因之一。目前的研究表明，PNPLA3（含3个蛋白素样磷脂酶结构域）的rs738409 G / C基因多态性与成人非酒精性脂肪性肝病有关[1，2]。但关于PNPLA3 rs738409 G / C基因的关系尚无共识。多态性和儿童NAFLD由于人群样本的差异。为此，进行了一项已发表研究的荟萃分析，以全面评估儿童中PNPLA3基因多态性与NAFLD之间的关系。从开始到2019年5月，我们搜索了MEDLINE，PubMed，EMBASE和CENTRAL数据库。根据纳入和排除标准，选择评估PNPLA3 rs738409 G / C基因多态性与儿童非酒精性脂肪性肝病之间关系的病例对照研究。使用随机效应模型量化PNPLA3 rs738409 G / C基因多态性与儿童NAFLD易感性之间的关联。使用固定效应模型量化PNPLA3 rs738409 G / C基因多态性与儿童NAFLD严重程度之间的关系。使用Stata 12.0软件进行数据分析。这项荟萃分析共包括9例病例对照研究，其中包含1173例NAFLD儿童和1792例健康对照的数据。五项研究比较了NAFLD儿童和非NAFLD健康人群。统计分析表明，在等位基因对比，显性，隐性和超显性模型中，PNPLA3基因多态性与儿童NAFLD显着相关（G vs C，OR = 3.343，95％CI = 1.524–7.334； GG + GC vs CC，OR = 3.157，95％CI = 1.446-6.892； GG vs GC + CC，OR = 5.692，95％CI = 1.941-16.689； GG + CC vs GC，OR = 2.756，95％CI = 1.729-4.392）。四个病例对照研究比较了非酒精性脂肪肝（NAFL）儿童和非酒精性脂肪性肝炎（NASH）儿童。结果显示，在隐性基因模型中，儿童的NPFLA3基因多态性也与NAFLD的严重程度显着相关（GG vs GC + CC，OR = 14.43，95％CI = 5.985-34.997）；Egger的检验没有明显的出版偏见。