Using analytical chemistry techniques such as nuclear magnetic resonance (NMR) spectroscopy and liquid or gas chromatography–mass spectrometry (LC/GC-MS), metabolomics allows detection of most endogenous and exogenous metabolites in a biological sample. Metabolomics has a wide range of applications, and has been employed in nutrition science, toxicology, environmental studies, and systems biology. Metabolomics is particularly useful in biomedical science, and has been used for diagnostic laboratory testing, identifying targets for drug development, and monitoring drug metabolism, mode of action, and toxicity. Despite its immense potential, metabolomics remains underutilized in the study of spontaneous animal diseases. Our aim was to comprehensively review the existing literature on the use of metabolomics in spontaneous veterinary diseases. Three databases were used to find journal articles that applied metabolomics in veterinary medicine. A screening process was then conducted to eliminate references that did not meet the eligibility criteria; only primary research studies investigating spontaneous animal disease were included; 38 studies met the inclusion criteria. The main techniques used were NMR and MS. All studies detected metabolite alterations in diseased animals compared with non-diseased animals. Metabolomics was mainly used to study diseases of the digestive, reproductive, and musculoskeletal systems. Inflammatory conditions made up the largest proportion of studies when articles were categorized by disease process. Following a comprehensive analysis of the literature on metabolomics in spontaneous veterinary diseases, we concluded that metabolomics, although in its early stages in veterinary research, is a promising tool regarding diagnosis, biomarker discovery, and in uncovering new insights into disease pathophysiology.

使用分析化学技术，如核磁共振 (NMR) 光谱和液相或气相色谱-质谱 (LC/GC-MS)，代谢组学可以检测生物样品中的大多数内源性和外源性代谢物。代谢组学具有广泛的应用，已被用于营养科学、毒理学、环境研究和系统生物学。代谢组学在生物医学科学中特别有用，已被用于诊断实验室测试、确定药物开发目标以及监测药物代谢、作用方式和毒性。尽管代谢组学具有巨大的潜力，但在自发性动物疾病的研究中仍未得到充分利用。我们的目的是全面审查有关代谢组学在自发性兽医疾病中使用的现有文献。三个数据库用于查找在兽医学中应用代谢组学的期刊文章。然后进行筛选过程以消除不符合资格标准的参考文献；仅包括调查自发性动物疾病的初级研究；38 项研究符合纳入标准。使用的主要技术是核磁共振和质谱。与未患病动物相比，所有研究均检测到患病动物的代谢物发生变化。代谢组学主要用于研究消化系统、生殖系统和肌肉骨骼系统的疾病。当文章按疾病过程分类时，炎症状况占研究的最大比例。在对自发性兽医疾病代谢组学的文献进行综合分析后，我们得出结论，代谢组学，