Paraoxonase 1 (PON1) is an inflammation marker associated with lipid oxidation and is used as a diagnostic marker in people. There is no information about the suitable substrate and analytic performance in cats, or its biological behavior compared with other inflammation markers. Our aims were to validate a paraoxon-based method to measure PON1 activity in feline serum, to assess stability of PON1 under different storage conditions and the impact of interfering elements, to determine a reference interval (RI) for healthy cats, and to correlate PON1 activity with 2 major acute-phase proteins. Intra- and inter-assay precision, accuracy, and RI were assessed using fresh serum. The same specimens were stored at room temperature, refrigerated, or frozen, and retested at defined intervals. Hemolysis, lipemia, and icterus were simulated to study interferences. PON1 results were compared to serum amyloid A (SAA) and alpha-1-acid glycoprotein (AGP) results. Analytical validation yielded precise and accurate results. PON1 activity is stable for up to 24 h at room temperature and up to 48 h at 4°C. Freezing at −20°C results in an increase after 72 h, with return to baseline values after 1 wk, that again increases after 6 mo. Only hyperlipemia interfered with PON1 activity. The RI based on 71 healthy cats was 58–154 U/L. PON1 activity was negatively correlated with AGP, but not with SAA. Serum PON1 activity can be measured accurately in cats, and it acts as a negative acute-phase protein.

对氧磷酶 1 (PON1) 是一种与脂质氧化相关的炎症标志物，在人体中用作诊断标志物。没有关于猫的合适底物和分析性能的信息，或其与其他炎症标志物相比的生物学行为。我们的目标是验证一种基于对氧磷的方法来测量猫血清中 PON1 的活性，评估 PON1 在不同储存条件下的稳定性和干扰元素的影响，确定健康猫的参考区间 (RI)，并关联 PON1 2 种主要急性期蛋白的活性。使用新鲜血清评估测定内和测定间的精密度、准确度和 RI。相同的样本在室温下储存、冷藏或冷冻，并在规定的时间间隔内重新测试。溶血、脂血、并模拟黄疸以研究干扰。将 PON1 结果与血清淀粉样蛋白 A (SAA) 和 α-1-酸性糖蛋白 (AGP) 结果进行比较。分析验证产生精确和准确的结果。PON1 活性在室温下可稳定长达 24 小时，在 4°C 下可长达 48 小时。-20°C 冷冻导致 72 小时后增加，1 周后返回基线值，6 个月后再次增加。只有高脂血症会干扰 PON1 的活性。基于 71 只健康猫的 RI 为 58-154 U/L。PON1 活性与 AGP 呈负相关，但与 SAA 无关。可以准确测量猫的血清 PON1 活性，它作为一种阴性急性期蛋白。PON1 活性在室温下可稳定长达 24 小时，在 4°C 下可长达 48 小时。-20°C 冷冻导致 72 小时后增加，1 周后返回基线值，6 个月后再次增加。只有高脂血症会干扰 PON1 的活性。基于 71 只健康猫的 RI 为 58-154 U/L。PON1 活性与 AGP 呈负相关，但与 SAA 无关。可以准确测量猫的血清 PON1 活性，它作为一种阴性急性期蛋白。PON1 活性在室温下可稳定长达 24 小时，在 4°C 下可长达 48 小时。-20°C 冷冻导致 72 小时后增加，1 周后返回基线值，6 个月后再次增加。只有高脂血症会干扰 PON1 的活性。基于 71 只健康猫的 RI 为 58-154 U/L。PON1 活性与 AGP 呈负相关，但与 SAA 无关。可以准确测量猫的血清 PON1 活性，它作为一种阴性急性期蛋白。