The lncRNA-599547 (619-nt in length) is identified in secondary hair follicle (SHF) of cashmere goat, but its functional roles in regulating the inductive property of dermal papilla cells (DPCs) remains unknown. We found that lncRNA-599547 had significantly higher expression in dermal papilla of cashmere goat SHF at anagen than its counterpart at telogen. The overexpression of lncRNA-599547 led to a significant increase of ALP and LEF1 expression in DPCs (p < 0.05), whereas, the siLncRNA-1 mediated silencing of lncRNA-599547 significantly down-regulated the expression of ALP and LEF1 in DPCs (p < 0.05). Based on biotin-labeled RNA pull-down assay, we found that lncRNA-599547 directly interacted with chi-miR-15b-5p in DPCs. Based on both overexpression and silencing analysis of lncRNA-599547, our results indicate that lncRNA-599547 promotes the expression of Wnt10b in DPCs but without modulating its promoter methylation level. Using the mRNA-3′UTR fragments of goat Wnt10b containing the predicted binding sites of chi-miR-15b-5p in Dual-luciferase Reporter Assays, we show that lncRNA-599547 modulates the expression of Wnt10b at the chi-miR-15b-5p mediated post-transcriptional level. Taken together, our results indicate that lncRNA-599547 sponges miR-15b-5p to positively regulate the expression of Wnt10 gene, and thereby contributes the inductive property of DPCs in cashmere goat.

在绒山羊的次生毛囊（SHF）中鉴定出lncRNA-599547（长度为619nt），但其在调节真皮乳头细胞（DPC）的诱导特性中的功能作用仍然未知。我们发现lncRNA-599547在成年羊绒中的绒毛山羊SHF的真皮乳头中的表达明显高于在端粒中的表达。lncRNA-599547的过表达导致DPC中ALP和LEF1表达的显着增加（p  <0.05），而siLncRNA-1介导的lncRNA-599547沉默显着下调了DPC中的ALP和LEF1的表达（p <0.05）。基于生物素标记的RNA下拉测定，我们发现lncRNA-599547与DPC中的chi-miR-15b-5p直接相互作用。基于lncRNA-599547的过表达和沉默分析，我们的结果表明lncRNA-599547促进了DPC中Wnt10b的表达，但没有调节其启动子甲基化水平。在双荧光素酶报告基因分析中，使用包含预期的chi-miR-15b-5p结合位点的山羊Wnt10b的mRNA-3'UTR片段，我们显示出lncRNA-599547调节了chi-miR-15b-上Wnt10b的表达5p介导的转录后水平。综上所述，我们的结果表明，lncRNA-599547可以使miR-15b-5p发挥海绵状作用，从而正向调节Wnt10基因的表达，从而有助于DPC在绒山羊中的诱导特性。