Atopic dermatitis (AD) is a relapsing, chronic, and inflammatory skin disorder. Its causes remain unclear. Here, we reported the first proteome study of the bacterial community in AD patients. Bacterial community in 7 patients and 1 healthy control using bottom-up proteomics were examined starting with in-solution digestion followed by purification steps with subsequent analysis using LC-MS/MS and ended with data processing and bioinformatic analysis. Overall, great bacterial changes between patient samples and healthy one were noticed with the presence of Staphylococcus aureus, Aeromonas hydrophila, and Shewanella species, and others that were present uniquely in patient samples suggesting their role in AD. Additionally, detection of some important proteins that trigger bacterial pathogenesis and the immune system such as enolase, glyceraldehyde-3-phosphate, Chaperone proteins DnaK and HtpG beside protein pathways needed for bacterial growth and pathogenesis like chaperones and folding catalysts; and Energy metabolism. These new findings of the microbiome and detected proteins could start a new era of proteomics to study the bacterial community as a whole and detect the way it interacts with each other and with the host.

Significance

This paper would represent a reference work for investigations on microbiota that present on AD, from both a microbiological and a functional proteomic point of view. We focused on analysisng bacteria community and proteins produced and its role in the disease, highlighting some functional characteristics of certain proteins and discussing its potential role in AD.

中文翻译：

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蛋白质组学分析与特应性皮炎有关的细菌群落。

特应性皮炎（AD）是一种复发性，慢性和炎症性皮肤病。其原因尚不清楚。在这里，我们报道了AD患者细菌群落的第一个蛋白质组研究。使用自下而上的蛋白质组学检查了7例患者和1个健康对照中的细菌群落，首先进行了溶液内消化，然后进行纯化步骤，随后使用LC-MS / MS分析，最后进行了数据处理和生物信息学分析。总体而言，在患者样本和健康样本之间发现了金黄色葡萄球菌，嗜水气单胞菌，和希瓦氏菌物种，以及在患者样本中独特存在的其他物种，表明它们在AD中的作用。另外，除了细菌生长和致病所需的蛋白质途径外，还检测出一些可引起细菌致病和免疫系统的重要蛋白质，例如烯醇酶，3-磷酸甘油醛，伴侣蛋白DnaK和HtpG；伴侣蛋白和折叠催化剂。和能量代谢。微生物组和检测到的蛋白质的这些新发现可能会开启蛋白质组学的新纪元，以研究整个细菌群落并检测其相互之间以及与宿主的相互作用方式。

意义

本文将从微生物学和功能蛋白质组学的角度为AD上存在的微生物群研究提供参考。我们重点分析细菌群落和产生的蛋白质及其在疾病中的作用，重点介绍某些蛋白质的某些功能特征，并讨论其在AD中的潜在作用。