Chimeras with improved properties can be designed by synergistically incorporating multiple proteins into one peptide chain. Such design enhances hydrolysis efficiency and lowers enzyme production costs for numerous applications, particularly in the fields of lignocellulosic biomass hydrolysis, plant-based industries, food additives, waste treatment, and biofuels. In this minireview, we summarize a brief, common approach to construct recombinant enzymes, especially in the Pichia pastoris host. We present the design strategies and the desired properties of chimeras and provide examples of recent studies in the area of lignocellulosic biomass hydrolysis. Subsequently, we describe the progress in structure prediction tools that facilitate the design of chimeric proteins toward identifying the best combinations of fusion partners and linkers.

中文翻译：

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用于木质纤维素生物质水解的重组嵌合酶

可以通过将多种蛋白质协同整合到一条肽链中来设计具有改进特性的嵌合体。这种设计提高了水解效率并降低了许多应用的酶生产成本，特别是在木质纤维素生物质水解、植物基工业、食品添加剂、废物处理和生物燃料领域。在这篇小综述中，我们总结了一种构建重组酶的简单、通用的方法，尤其是在毕赤酵母宿主中。我们介绍了嵌合体的设计策略和所需的特性，并提供了木质纤维素生物质水解领域的最新研究实例。随后，我们描述了结构预测工具的进展，这些工具有助于设计嵌合蛋白，以确定融合伙伴和接头的最佳组合。