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Transoperative glycemia in pets: validating old ones, and presenting lip mucosa as new sampling site.
Domestic Animal Endocrinology ( IF 2.1 ) Pub Date : 2020-08-17 , DOI: 10.1016/j.domaniend.2020.106540
P B Costa 1 , M L Ricarti 2 , C A A Augustavo 2 , L S Santos 3 , L Z Crivellenti 2 , S Borin-Crivellenti 4
Affiliation  

The aim of our study was to investigate the viability and validity of blood sampling from the upper lip mucosa in healthy dogs and cats for monitoring transoperative glycemia and compare the results with those obtained from samples taken from previously described blood sampling sites for determination of glycemia using a glucose meter. Blood glucose (BG) levels were determined in samples taken from the upper lip mucosa of 24 dogs and 31 cats undergoing neutering or spaying surgeries. These values were compared to those of samples obtained from other sites previously described for capillary blood glucose monitoring (marginal ear vein, carpal pad in dogs, metacarpal pad in cats) using a glucose meter. Additionally, BG from peripheral venous blood was determined using a glucose meter, and the gold standard enzymatic colorimetric assay. The clinical reliability of BG values taken from lip mucosa and from all the other BG values measured by the glucose meter was evaluated using the error grid analysis modified by Parkes et al (2000). The upper lip mucosa was an easily accessible site for the obtainment of appropriate blood samples, and glucose levels read in these samples correlated positively with glycemic values read in blood samples from all other sites in dogs and cats. All BG made using glucose meters taken from all sites were within the clinically acceptable range when compared with enzymatic colorimetric assay (gold standard), and were analytically accurate according to the error grid (zones A and B). All blood sampling sites described in this work can be used to assess transoperative glycemia. The upper lip mucosa is a viable blood sampling site for precise monitoring of transoperative glycemia in healthy dogs and cats and shows promise for alternative blood glucose monitoring.



中文翻译:

宠物的跨手术血糖:验证旧的,并将唇粘膜作为新的采样点。

我们研究的目的是调查健康犬猫上唇粘膜采血监测经手术血糖的可行性和有效性,并将结果与​​从先前描述的采血部位采集的样本获得的结果进行比较,以使用血糖仪。从接受绝育或绝育手术的 24 只狗和 31 只猫的上唇粘膜样本中测定血糖 (BG) 水平。使用血糖仪将这些值与从先前描述的用于毛细血管血糖监测的其他部位(耳缘静脉、狗的腕垫、猫的掌垫)获得的样本进行比较。此外,使用血糖仪和金标准酶促比色法测定外周静脉血的 BG。使用由 Parkes 等人 (2000) 修改的误差网格分析来评估取自唇粘膜的 BG 值和由血糖仪测量的所有其他 BG 值的临床可靠性。上唇粘膜是获取适当血样的容易接近的部位,并且这些样品中读取的葡萄糖水平与来自狗和猫的所有其他部位的血样中读取的血糖值呈正相关。与酶比色法(金标准)相比,使用从所有地点采集的血糖仪制成的所有 BG 都在临床可接受的范围内,并且根据误差网格(区域 A 和 B)在分析上是准确的。这项工作中描述的所有采血点都可用于评估跨手术血糖。

更新日期:2020-08-17
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