Journal of Applied Genetics ( IF 2.4 ) Pub Date : 2020-08-17 , DOI: 10.1007/s13353-020-00574-4 Mohtaram Mahmoudieh 1 , Mohamad Roff Mohd Noor 2 , Jennifer Ann Harikrishna 1 , Rofina Yasmin Othman 1
The study describes results of a survey of tomato fields for the presence of begomoviruses from different regions of Peninsular Malaysia. An ORF-based (C2 and C3) study was performed to determine the distribution of begomoviruses associated with a severe leaf curl disease in tomato-growing areas of Peninsular Malaysia. Viral DNA was isolated from symptomatic tomato plants, and begomovirus association was confirmed by PCR using DNA-A degenerate primers. The C2 and C3 sequences of the putative begomoviruses were similar to two corresponded ORFs of different geographically separated strains of begomoviruses: Pepper yellow leaf curl Indonesia virus and Tomato yellow leaf curl Kanchanaburi virus. The present study also identified a unique isolate, Ageratum yellow vein Malaysia virus (AYVMV) among above mentioned survey. It has a single-stranded DNA component and its associated betasatellite. The single-stranded DNA component is consisting of 2750 nt with six open reading frames and an organization resembling that of monopartite geminiviruses. The full length of viral single-stranded DNA component genome obtained using next generation sequencing (NGS) showed the highest sequence identity (99%) with Ageratum yellow vein virus (AYVV-BA). The betasatellite component genome obtained by NGS has 1342 nt and showed the highest sequence identity (91%) with the Pepper yellow leaf curl betasatellite. Following ICTV guidelines, Ageratum yellow vein Malaysia virus was assigned the abbreviation AYVMV with sequence and phylogenetic analysis indicating that it might have evolved by recombination of two or more viral ancestors.
中文翻译:
鉴定和表征在马来西亚感染番茄茄的begomovirus中的香叶黄脉马来西亚病毒(AYVMV)和相关的β卫星。
这项研究描述了对番茄田的调查结果,该番茄田中存在来自马来西亚半岛不同地区的Begomoviruses病毒。进行了基于ORF的研究(C2和C3),以确定与严重叶片卷曲病相关的begomoviruses在马来西亚半岛番茄种植区的分布。从有症状的番茄植株中分离出病毒DNA,并使用DNA-A简并引物通过PCR确认了begomovirus缔合。推定的begomovirus的C2和C3序列与begomovirus的不同地理分离株的两个对应的ORF相似:辣椒黄叶卷曲印度尼西亚病毒和番茄黄叶卷曲北碧病毒。本研究还鉴定出一种独特的分离物,香ger黄脉马来西亚病毒(AYVMV)在上述调查中。它具有单链DNA成分及其相关的β卫星。单链DNA组件由2750 nt的核苷酸和六个开放阅读框组成,其组织类似于单倍体双生病毒。使用下一代测序(NGS)获得的病毒单链DNA组分基因组的全长与Ageratum黄脉病毒(AYVV-BA)表现出最高的序列同一性(99%)。由NGS获得的β卫星组分基因组具有1342 nt,与辣椒黄色叶片卷曲的β卫星显示最高的序列同一性(91%)。根据ICTV指南,A香黄脉马来西亚病毒 被赋予缩写AYVMV,并进行了序列和系统发育分析,表明它可能是由于两个或多个病毒祖先的重组而进化的。