Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug, due to its wide distribution and multidrug resistance. To characterize effects of a newly identified plantaricin GZ1-27 on MRSA, transcriptomic and proteomic profiling of MRSA strain ATCC43300 was performed in response to sub-MIC (16 μg/mL) plantaricin GZ1-27 stress. In total, 1090 differentially expressed genes (padj < 0.05) and 418 differentially expressed proteins (fold change > 1.2, p < 0.05) were identified. Centralized protein expression clusters were predicted in biological functions (biofilm formation, DNA replication and repair, and heat-shock) and metabolic pathways (purine metabolism, amino acid metabolism, and biosynthesis of secondary metabolites). Moreover, a capacity of inhibition MRSA biofilm formation and killing biofilm cells were verified using crystal violet staining, scanning electron microscopy, and confocal laser-scanning microscopy. These findings yielded comprehensive new data regarding responses induced by plantaricin and could inform evidence-based methods to mitigate MRSA biofilm formation.

耐甲氧西林的金黄色葡萄球菌（MRSA）由于其广泛的分布和多药耐药性而成为令人担忧的超级细菌。为了表征新鉴定的plant菌素GZ1-27对MRSA的作用，响应亚MIC（16μg/ mL）ari菌素GZ1-27胁迫，对MRSA株ATCC43300进行了转录组和蛋白质组学分析。总共有1090个差异表达的基因（padj  <0.05）和418个差异表达的蛋白质（倍数变化> 1.2，p <0.05）。在生物学功能（生物膜形成，DNA复制和修复以及热休克）和代谢途径（嘌呤代谢，氨基酸代谢和次生代谢产物的生物合成）中预测了集中的蛋白表达簇。此外，使用结晶紫染色，扫描电子显微镜和共聚焦激光扫描显微镜证实了抑制MRSA生物膜形成和杀死生物膜细胞的能力。这些发现产生了关于plant那霉素诱导的反应的全面的新数据，并且可以为减轻MRSA生物膜形成的循证方法提供信息。