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An Effective Acid-Base-Induced Liquid-Liquid Microextraction Based on Deep Eutectic Solvents for Determination of Testosterone and Methyltestosterone in Milk.
Journal of Chromatographic Science ( IF 1.3 ) Pub Date : 2020-08-14 , DOI: 10.1093/chromsci/bmaa051
Xiao Li 1, 2, 3 , Tao Yuan 3 , Ting Zhao 2, 3 , Xiaomei Wu 3 , Yaling Yang 1
Affiliation  

An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5–100 μg mL−1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL−1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%.

中文翻译:

一种基于深共晶溶剂的有效酸碱诱导的液体微萃取技术,用于测定牛奶中的睾丸激素和甲基睾丸激素。

建立了一种由酸碱诱导的低共熔溶剂液-液微萃取(DES-ABLLME)结合高效液相色谱法测定睾丸激素和甲基睾丸激素的环保方法。由薄荷醇:月桂酸:癸酸(3:1:1)组成的深共熔溶剂(DES)既可以充当氢键供体,也可以充当氢键受体。在这种方法中,氨溶液(NH 3 •H 2O)用作乳化剂,在萃取过程中与DES发生反应,生成盐并形成乳白色溶液,从而实现了较高的萃取效率。盐酸被用作相分离剂,以改变乳化状态并促进萃取剂与水相的分离。优化了一系列参数,包括DES和乳化剂的体积,葡萄糖浓度以及盐酸体积。该方法在0.5–100μgmL -1范围内呈线性,相关系数(R)为0.9999,检测限为0.067和0.2μgmL -1分别用于睾丸激素和甲基睾丸激素。该方法用于牛奶样品中睾丸激素和甲基睾丸激素的分析,回收率在89.2%至108.2%之间。
更新日期:2020-09-29
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