We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularly permuted 178,039-bp dsDNA molecule with a GC content of 43.4%. It has been predicted to contain 282 protein-coding genes and two tRNA genes, tRNA-Met and tRNA-Gly. Using bioinformatics approaches, 99 (36%) of the vB_ButM_GuL6 genes were assigned a putative function. Genome-wide comparisons and phylogenetic analysis indicated that vB_ButM_GuL6 represents a new species of the subfamily Tevenvirinae and is most closely related to Escherichia virus RB43. These phages, together with Cronobacter phages Miller, CfP1, and IME-CF2, likely form a new genus within the subfamily Tevenvirinae.

我们在座的裂解噬菌体，vB_ButM_GuL6，这是从分离到的第一个病毒的完整基因组序列的分析结果布丘氏。电子显微镜显示vB_ButM_GuL6属于肌病毒科，属于拟病毒科。vB_ButM_GuL6的基因组是一个线性的，环状排列的178,039-bp dsDNA分子，GC含量为43.4％。预计它包含282个蛋白质编码基因和两个tRNA基因，tRNA-Met和tRNA-Gly。使用生物信息学方法，向vB_ButM_GuL6基因中的99个（36％）分配了假定功能。全基因组比较和系统发育分析表明vB_ButM_GuL6代表Tevenvirinae亚科的一个新物种与埃希氏菌RB43关系最密切。这些噬菌体，连同坂噬菌体-米勒，CFP1和IME-CF2，可能形成亚科新属Tevenvirinae。