Ficus krishnae which belongs to the family Moraceae is an endangered plant species with medicinal potential. Due to over-exploitative commercial use, it has become endangered hence demands immediate conservation. Application of in vitro strategies in conservation of plants is an efficient technique to produce true-to-type plantlets. In the present study, nodal segment explants were used as induction of shoots on Murashige & Skoog (MS) medium fortified with various concentrations of benzylaminopurine (BAP) in combination with 0.5 mg/l naphthalene acetic acid (NAA) to determine optimum levels of BAP and NAA for multiple shoot formation. Cytokinins, BAP and kinetin (Kn) were tested for shoot regeneration from nodal and axillary shoot tip explants. Among all the treatments BAP and Kn tested BAP at 4.0 mg/l and at 2.5 mg/l responds optimum in shoot regeneration from nodal explants and axillary shoot tip, respectively. Average number of sprouted shoots per explant and length of these shoots were determined after three weeks of explant inoculation. Axillary- shoot tip explants show best results of callus induction at 2 mg/l 2,4 diphenoxy acetic acid (2, 4-D) with a response percentage of 83.33%. Moreover, it was also noted that among the different concentrations of indole butyric acid (IBA) tested, IBA at the concentration of 1.5 mg/l resulted best in inducing the maximum number (12.66 ± 0.33) of in vitro adventitious roots from the callus. In this study combination of BAP + NAA (4.0 + 0.5) mg/l and 2 mg/l (2,4-D) resulted better in shoot induction and callus formation respectively in F. Krishnae.

中文翻译：

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榕树的离体再生，愈伤组织诱导和发根：一种珍稀濒危植物

属于桑科（Moraceae）的克里氏榕（Ficus krishnae）是具有药用潜力的濒危植物。由于过度开发性商业用途，它已经濒临灭绝，因此需要立即加以保护。体外应用植物保护策略是生产真型小植株的有效技术。在本研究中，将节段外植体用于在Murashige＆Skoog（MS）培养基上诱导枝条，Murashige＆Skoog（MS）培养基中添加了各种浓度的苄基氨基嘌呤（BAP）和0.5 mg / l萘乙酸（NAA），以确定最佳BAP水平和NAA来形成多个芽。测试了细胞分裂素，BAP和激动素（Kn）在结节和腋生芽尖外植体中的芽再生。在所有处理中，BAP和Kn测试的BAP浓度分别为4.0 mg / l和2.5 mg / l时，分别对结节外植体和腋生芽梢的再生具有最佳响应。在外植体接种三周后，确定每个外植体的平均发芽芽数和这些芽的长度。腋生茎尖外植体在2 mg / l 2,4二苯氧基乙酸（2，4-D）上显示出最佳的愈伤组织诱导结果，响应百分率为83.33％。此外，还应注意的是，在测试的不同浓度的吲哚丁酸（IBA）中，浓度为1.5 mg / l的IBA诱导的最大数量（12.66±0.33）最好。愈伤组织的体外不定根。在这项研究中，BAP + NAA（4.0 + 0.5）mg / l和2 mg / l（2,4-D）的组合分别导致F. Krishnae的芽诱导和愈伤组织形成更好。