High laser powers are common practice in single-molecule localization microscopy to speed up data acquisition. Here we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong trade-off between imaging speed and quality and present optimized imaging protocols for high-throughput, multicolor and three-dimensional single-molecule localization microscopy with greatly improved resolution and effective labeling efficiency.

中文翻译：

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为单分子定位显微镜优化成像速度和激发强度。

高激光功率是单分子定位显微镜中的常见做法，可加快数据采集速度。在这里，我们系统地量化了激发强度如何影响定位精度和标记密度，这是决定数据质量的两个主要因素。我们发现了成像速度和质量之间的重大折衷，并且针对高通量，多色和三维单分子定位显微镜提出了优化的成像方案，从而大大提高了分辨率和有效的标记效率。