The aim of this study was to evaluate the effect of chlorhexidine and/or ethanol application on the bond strength of two universal adhesive systems. 36 extracted human teeth were used in this study. Standard Class I cavities (4 × 3 × 3mm) were prepared and divided into two groups according to the adhesive systems used. Each group was subdivided into three subgroups to be treated with chlorhexidine, ethanol, or unexposed to any solution. Group I; Universal bond I was applied without any treatment. Group II; 2% chlorhexidine was applied, before the application of universal bond I. Group III; 100% ethanol was applied, before the application of universal bond I. Group IV; Universal bond II was applied without any treatment. Group V; 2% chlorhexidine was applied, before the application of universal bond II. Group VI; 100% ethanol was applied, before the application of universal bond II. Then, teeth were restored with a nano-hybrid resin composite then subjected to thermo-cycling (10,000 cycles, 5–55 °C). All teeth were exposed to 50,000 cycles of loading in a chewing simulator. Twelve sticks from each group were obtained using a cutting machine. Sticks were subjected to a microtensile bond strength. After procedures, fractured surfaces were observed under a stereomicroscope to determine the mode of failure. Hybrid layer of each group was evaluated under a scanning electron microscope (SEM). Data were submitted to the Kruskal Wallis test. Significance level was set at 5%.No statistically significant differences were shown in all groups (p > 0.05). The lowest bond strength value was obtained in group II (9.76), the highest bond strength value was determined in group IV (20.57). The study concluded that additional chlorhexidine or ethanol treatment may not influence directly the bond strength of universal adhesives when using a single-step self etch mode of universal adhesives.

这项研究的目的是评估洗必泰和/或乙醇对两种通用胶粘剂体系粘合强度的影响。在这项研究中使用了36颗人类牙齿。准备了标准的I类腔（4×3×3mm），并根据所使用的粘合剂体系分为两组。每组再分为三个亚组，用洗必太，乙醇或不暴露于任何溶液中进行治疗。第一组；通用键I无需任何处理即可使用。第二组 在施加通用键I之前，施加2％的洗必太。在施加通用键I之前，施加100％乙醇。通用键II无需任何处理即可使用。第五组 在施加通用键II之前，施加2％的洗必太。第六组 使用100％乙醇，在申请通用债券之前II。然后，用纳米混合树脂复合材料修复牙齿，然后进行热循环（10,000个循环，5–55°C）。在咀嚼模拟器中，所有牙齿都承受了50,000个负载循环。使用切割机从每个组中获得十二根棍棒。棒经受微拉伸粘合强度。手术后，在立体显微镜下观察破裂的表面，以确定破坏的方式。在扫描电子显微镜（SEM）下评估每组的杂化层。数据已提交给Kruskal Wallis测试。显着性水平设置为5％。所有组均未显示统计学显着性差异（5–55°C）。在咀嚼模拟器中，所有牙齿都承受了50,000个负载循环。使用切割机从每个组中获得十二根棍棒。棒经受微拉伸粘合强度。手术后，在立体显微镜下观察破裂的表面，以确定破坏的方式。在扫描电子显微镜（SEM）下评估每组的杂化层。数据已提交给Kruskal Wallis测试。显着性水平设置为5％。所有组均未显示统计学显着性差异（5–55°C）。在咀嚼模拟器中，所有牙齿都承受了50,000个负载循环。使用切割机从每个组中获得十二根棍棒。棒经受微拉伸粘合强度。手术后，在立体显微镜下观察破裂的表面，以确定破坏的方式。在扫描电子显微镜（SEM）下评估每组的杂化层。数据已提交给Kruskal Wallis测试。显着性水平设置为5％。所有组均未显示统计学显着性差异（在立体显微镜下观察断裂的表面以确定破坏的方式。在扫描电子显微镜（SEM）下评估每组的杂化层。数据已提交给Kruskal Wallis测试。显着性水平设置为5％。所有组均未显示统计学显着性差异（在立体显微镜下观察断裂的表面以确定破坏的方式。在扫描电子显微镜（SEM）下评估每组的杂化层。数据已提交给Kruskal Wallis测试。显着性水平设置为5％。所有组均未显示统计学显着性差异（p  > 0.05）。在组II中获得最低的结合强度值（9.76），在组IV中获得最高的结合强度值（20.57）。研究得出结论，使用通用粘合剂的单步自蚀刻模式时，额外的洗必太或乙醇处理可能不会直接影响通用粘合剂的粘合强度。