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Biological control of Botrytis cinerea, the causal agent of rose gray mold disease by antagonistic fungi
International Journal of Pest Management ( IF 1.5 ) Pub Date : 2020-08-17
Mohammad Reza Safari Motlagh, Nastaran Jafari

In the present study, 15 fungal isolates were found. The antagonistic potential of seven of these, including Trichoderma harzianum, T. viride, T. virens, Aspergillus fumigatus, A. niger, Aspergillus sp., and Drechslera triseptata were proved under in vitro and greenhouse conditions. In the dual culture method, T. virens and T. viride (65.51%), in the volatile compounds method, T. harzianum (66.66%), and in the non-volatile compounds method, T. virens (63%), had the highest inhibitory effect on fungal mycelial growth. In the slide culture method, all the studied isolates prevented the progress of pathogenic fungal hyphae and in some deformed the hyphae. All the inoculated fungi reduced the disease severity of B. cinerea, among which T. virens was the most effective fungus, reducing the disease severity by 26%. Also, the examination of height, fresh weight, and dry weight of rose plants under greenhouse conditions revealed that the application of T. virens increased these characteristics in the presence of the pathogen. The application of other fungi tested also increased these characteristics in the presence of B. cinerea.



中文翻译:

灰霉病病原菌拮抗真菌灰葡萄孢的生物防治

在本研究中,发现了15种真菌分离株。在体外和温室条件下证明了其中的7种具有拮抗作用,其中包括哈茨木霉Trichoderma harzianum)维尔纽斯T. viride)维尔纽斯T. virens)烟曲霉Aspergillus fumigatus)曲霉A.niger)曲霉(Aspergillus sp。)和Drechslera triseptata。在双培养方法,绿木霉绿色木霉(65.51%),在易失性的化合物的方法,哈茨木霉(66.66%),和在非挥发性化合物的方法,绿木霉(63%),对真菌菌丝生长具有最高的抑制作用。在载玻片培养方法中,所有研究的菌株均阻止了病原性真菌菌丝的进展,并且在某些情况下使菌丝变形。所有接种的真菌均降低了灰葡萄孢菌的病情严重程度,其中最有效的真菌是维尔纽斯菌,使病情严重程度降低了26%。而且,在温室条件下对玫瑰植物的高度,鲜重和干重的检查表明,在病原体存在下,施以维氏梭菌可增加这些特性。在B. cinerea的存在下,其他测试真菌的应用也增加了这些特性。

更新日期:2020-08-17
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