Numerous investigations on the antioxidant properties of different plant tissue extracts are available in literature, but few studies on the effect of thermally modified wood on secondary metabolites were carried out up to now. This study highlights the influence of the thermal modification of Castanea sativa Mill. wood on total content of antioxidant compounds and consequently on its antioxidant activity. In addition, a chemical profile by Gas Chromatography–Mass Spectrometry (GC–MS) of the extractives was carried out. Wood of chestnut, thermally modified at 180 °C for 3 h, was used to obtain wood meal which was subjected to different extraction techniques, as maceration extraction (ME), ultrasound assisted extraction (UAE) and accelerated solvent extraction (ASE). The total content of principal antioxidant compounds, such as polyphenols, flavonoids and tannins, as well as the evaluation of antioxidant capacity by using different in vitro assays were determined. Relative Antioxidant Capacity Index (RACI), which is used to compare all antioxidant parameters, has also been applied. The study demonstrated a positive influence on chemical compounds present in C. sativa Mill wood originating from the thermal modification process. Thus, is possible to consider thermal modification as a promising strategy to improve the antioxidant activity of chestnut wood extractives.

中文翻译：

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热改性和提取工艺对板栗（Castanea sativa Mill。）木材产量，抗氧化能力和植物化学特性的影响

关于不同植物组织提取物的抗氧化性能的大量研究可在文献中找到，但是迄今为止，关于热改性木材对次生代谢产物的影响的研究很少。这项研究强调了栗木热变质的影响。木材中抗氧化剂化合物的总含量，因此也涉及其抗氧化活性。此外，通过气相色谱-质谱法（GC-MS）对提取物进行了化学分析。板栗木材在180°C的温度下进行了3小时的热改性，用于获得木粉，该木粉采用了浸软提取（ME），超声辅助提取（UAE）和加速溶剂提取（ASE）的不同提取技术。主要抗氧化剂化合物（例如多酚）的总含量 确定了类黄酮和丹宁酸的含量，以及通过使用不同的体外测定方法评估抗氧化能力的方法。相对抗氧化剂容量指数（RACI），用于比较所有抗氧化剂参数，也已应用。这项研究证明了对源自热修饰过程的紫花苜蓿木材中存在的化学化合物的积极影响。因此，可以将热改性视为改善栗木提取物抗氧化活性的有前途的策略。苜蓿木材起源于热改性过程。因此，可以将热改性视为改善栗木提取物抗氧化活性的有前途的策略。苜蓿木材源自热改性过程。因此，可以将热改性视为改善栗木提取物抗氧化活性的有前途的策略。