Abstract To solve the bio-inertness of widely used polypropylene (PP) mesh for treating pelvic floor dysfunction (PFD), a novel strategy of incorporation with elastin gene-transfected bone marrow stem cells (BMSCs) and antibacteria drug-loaded polylactic acid (PLA) nanofibrous mat covering layer was proposed to overcome the limitation of the pristine PP mesh. Then, a series of physicochemical and in vitro experiments were applied to investigate the improvement of the as-prepared material. The elastin protein expression was proved to be upregulated without obvious cytotoxicity influence after the gene transfection and also improved the cell migration rate. In addition, the antibacteria drug-loaded PLA nanofibrous mat on the PP mesh could efficiently inhibit bacteria and showed no significant impact on cell adhesion and proliferation. Thus, we believe that the incorporation of the elastin gene-transfected BMSCs and nanofiber-coated PP mesh would be a potential candidate in the application of female PFD.

中文翻译：

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抗菌聚合物纳米纤维涂层和高弹性蛋白表达 BMSCs 结合聚丙烯网用于加速女性盆底功能障碍的愈合

摘要 为了解决广泛使用的聚丙烯 (PP) 网片治疗盆底功能障碍 (PFD) 的生物惰性问题，提出了一种将弹性蛋白基因转染的骨髓干细胞 (BMSCs) 和抗菌载药聚乳酸 (PLA ) 纳米纤维垫覆盖层被提出来克服原始 PP 网的局限性。然后，应用一系列理化和体外实验来研究所制备材料的改进。证明基因转染后弹性蛋白表达上调，无明显细胞毒性影响，也提高了细胞迁移率。此外，PP网上载有抗菌药物的PLA纳米纤维垫可以有效抑制细菌，对细胞粘附和增殖没有显着影响。因此，