Abstract

Objective The aim of this study was to determine the time (T_max) to the maximum concentration (C_max) of amikacin sulphate in synovial fluid of the radiocarpal joint (RCJ) following cephalic intravenous regional limb perfusion (IVRLP) using 2 g of amikacin sulphate.

Methods Cephalic IVRLP was performed with 2 g of amikacin sulphate diluted in 0.9% NaCl to a total volume of 100 mL in six healthy adult mixed breed mares. An Esmarch's rubber tourniquet was applied for 30 minutes and the antibiotic solution was infused through a 23-gauge butterfly catheter. Synovial fluid was collected from the RCJ prior to the infusion and at 5, 10, 15, 20, 25 and 30 minutes after completion of IVRLP. The tourniquet was removed after the last arthrocentesis. Synovial fluid amikacin sulphate concentrations were determined by liquid chromatography/tandem mass spectrometry.

Results The calculated mean T_max occurred at 15 minutes (range: 10–20 minutes) post-perfusion. The highest synovial fluid amikacin sulphate concentration was noted at 10 minutes in 2 horses, 15 minutes in 2 horses and 20 minutes in 2 horses. The highest mean concentration was 1023 µg/mL and was noted at 20 minutes. Synovial mean concentrations were significantly different between 15 and 30 minutes.

Clinical Significance In this study no T_max occurred after 20 minutes; thus, 30 minutes of tourniquet application time appear to be excessive. The 20 minutes duration of tourniquet application appears sufficient for the treatment of the RCJ in cephalic IVRLP using 2 g amikacin sulphate in a total volume of 100 mL.

Authors' Contributions

Kajsa Gustafsson, Amos J. Tatz, Roee Dahan, and Gal Kelmer contributed to the conception of study. Kajsa Gustafsson, Amos J. Tatz, Roee Dahan, Malka Britzi, and Gal Kelmer contributed to acquisition of data. Kajsa Gustafsson, Amos J. Tatz, Roee Dahan, Malka Britzi, Stefan Soback, Gila A. Sutton, and Gal Kelmer contributed to data analysis and interpretation. All authors drafted, revised and approved the submitted manuscript.

中文翻译：

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站立马的头脑静脉区域四肢灌注后，达到前腕掌关节中阿米卡星浓度峰值的时间。

摘要

目的 本研究的目的是确定使用2 g阿米卡星进行头部静脉区域性肢体灌注（IVRLP）后，carp腕关节（RCJ）滑液中硫酸阿米卡星达到最大浓度（C _max）的时间（T _max）。硫酸盐。

方法 在6只健康的成年混合母马中，用2 g溶于0.9％NaCl的阿米卡星硫酸盐进行总剂量为100 mL的头孢IVRLP。施加一个Esmarch的橡胶止血带30分钟，然后通过23号蝴蝶导管注入抗生素溶液。在输注之前以及完成IVRLP后5、10、15、20、25和30分钟时，从RCJ收集滑液。上一次关节穿刺术结束后，止血带被拔除。通过液相色谱/串联质谱法测定滑液中阿米卡星硫酸盐的浓度。

结果 计算出的平均T _max发生在灌注后15分钟（范围：10–20分钟）。滑膜液中硫酸阿米卡星硫酸盐的最高浓度在2匹马中10分钟出现，在2匹马中15分钟出现，在2匹马中20分钟出现。最高平均浓度为1023 µg / mL，记录在20分钟时。滑膜平均浓度在15至30分钟之间显着不同。

临床意义 在本研究中，20分钟后未出现T _max；而在20分钟后未出现T _max。因此，施加30分钟的止血带时间过长。施加20分钟的止血带持续时间足以使用总体积为100 mL的2 g硫酸阿米卡星治疗头颅IVRLP中的RCJ。

作者的贡献

Kajsa Gustafsson，Amos J. Tatz，Roee Dahan和Gal Kelmer为研究构想做出了贡献。Kajsa Gustafsson，Amos J. Tatz，Roee Dahan，Malka Britzi和Gal Kelmer促成了数据的获取。Kajsa Gustafsson，Amos J. Tatz，Roee Dahan，Malka Britzi，Stefan Soback，Gila A.Sutton和Gal Kelmer都为数据分析和解释做出了贡献。所有作者都起草，修改和批准了提交的手稿。