The present study was conducted to determine the effect of curcumin supplement diet (CSD) on growth, non‐specific immunity and pro‐inflammatory cytokine gene expression in Labeo rohita fingerlings. 60‐day (0–45 days pre‐challenge and 15 days post challenge; Aeromonas hydrophila) experiment was conducted for with four treatment groups: T1 (0.5% curcumin level), T2 (1% curcumin level), T3 (1.5% curcumin level), T4 (2% curcumin level) and a control group (0% curcumin level). Kidney tissue samples were collected for RT‐PCR from all the treatment groups at 15 days of interval. After 45 days of feeding, a sub‐sample of fish (15 fish from each replicate) were challenged with a virulent strain of A. hydrophila. The mRNA expression levels of immune‐related genes, viz. tumour necrosis factor‐alpha (TNF‐α), interleukin‐1β (IL‐1β) and Toll‐like receptor‐22 (TLR‐22), were evaluated along with nitro blue tetrazolium (NBT) assay and lysozyme activity. TNF‐α mRNA expression levels were significantly lowered in CSD treatment with the lowest level observed in the T₂ treatment group, followed by T₁ during pre‐challenging period respectively. The IL‐1β and TLR‐22 expression levels also followed the similar trend in different treatment groups. Similarly, NBT and serum lysozyme activities were significantly different among treatment groups. Fish fed with 1.5% level curcumin diet (T3) had significantly highest relative percentage survival (63.64%) compared to the rest of the treatment groups. The results suggested that the diet supplemented with 1.5% curcumin level provides protective effect against A. hydrophila in L. rohita by increasing immunity and reducing the activity of pro‐inflammatory genes.

中文翻译：

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姜黄素补充饮食：罗汉果鱼种的生长增强和促炎细胞因子的表达下调

本研究旨在确定姜黄素补充饮食（CSD）对生唇 鱼生长，非特异性免疫和促炎细胞因子基因表达的影响 。针对四个治疗组进行了60天（攻击前0-45天和攻击后15天；嗜水气单胞菌）实验：T1（姜黄素水平为0.5％），T2（姜黄素水平为1％），T3（姜黄素为1.5％）水平），T4（姜黄素水平为2％）和对照组（姜黄素水平为0％）。每隔15天从所有治疗组收集肾脏组织样本进行RT-PCR。后喂养45天，鱼（从每个重复15鱼）的子样品用的强毒力株攻击嗜水。免疫相关基因的mRNA表达水平，即。评估了肿瘤坏死因子α（TNF-α），白细胞介素1β（IL-1β）和Toll样受体22（TLR-22）以及硝基蓝四唑（NBT）分析和溶菌酶活性。在CSD治疗中，TNF-αmRNA表达水平显着降低，在T ₂治疗组中最低，随后是T ₁在挑战前期。在不同的治疗组中，IL-1β和TLR-22的表达水平也遵循相似的趋势。同样，治疗组之间的NBT和血清溶菌酶活性也存在显着差异。与其余治疗组相比，饲喂1.5％水平姜黄素饮食（T3）的鱼具有相对最高的存活率（63.64％）。结果表明，在补充有1.5％姜黄素水平的饮食提供对保护作用嗜水在L.鲮 通过增加免疫力和降低促炎性基因的活性。