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Temporal dynamics of innate and adaptive immune responses in broiler birds to oral delivered chitosan nanoparticle-based Salmonella subunit antigens.
Veterinary Immunology and Immunopathology ( IF 1.8 ) Pub Date : 2020-08-17 , DOI: 10.1016/j.vetimm.2020.110111
Y Han 1 , S Renu 1 , J Schrock 1 , K Y Acevedo-Villanuev 2 , B Lester 2 , R K Selvaraj 2 , G J Renukaradhya 1
Affiliation  

Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) infection of poultry causes a significant risk to public health through contamination of meat and eggs. Current Salmonella vaccines have failed to provide strong mucosal immunity in the intestines to reduce Salmonella shedding and food contamination. Considering the short lifespan of broilers, an easy-to-deliver, safe and effective Salmonella vaccine is urgently needed. Our goal in this study was to demonstrate the ability of chitosan nanoparticle (CNP) vaccine delivery platform in activating immune response to Salmonella antigens in broilers inoculated orally. In an initial study, soluble whole antigen of SE entrapped in CNP was inoculated but the specific immune responses were poor. Therefore, the CNP entrapped immunogenic outer membrane proteins (OMP) and flagellin (FLA) of SE and surface conjugated with FLA [CNP-(OMP + FLA)] was developed. In broilers inoculated orally with CNP-(OMP + FLA) formulation once or twice, we monitored the temporal expression of innate immune molecules and antigen specific lymphocyte proliferation. In the cecal tonsils of CNP-(OMP + FLA) inoculated birds, we observed enhanced expression of mRNA coding Toll-like receptors (TLRs)- 1, 4, 5, and 7, especially at dpv 21. In addition, both OMP and FLA specific lymphocytes proliferation at dpv 7 and 21 by CNP-(OMP + FLA) were enhanced in the spleen. In conclusion, CNP-(OMP + FLA) formulation augmented both innate and lymphocyte responses in orally inoculated broilers. Further studies are needed to determine the candidate subunit CNP vaccine’s efficacy in a challenge trial.



中文翻译:

肉鸡对口服递送的基于壳聚糖纳米颗粒的沙门氏菌亚单位抗原的先天性和适应性免疫反应的时间动态。

沙门氏菌血清型肠炎(S.肠炎,SE)家禽感染引起,通过肉类和鸡蛋的污染显著公共卫生风险。当前的沙门氏菌疫苗未能在肠道中提供强大的粘膜免疫力,以减少沙门氏菌的脱落和食物污染。考虑到肉鸡寿命短,迫切需要一种易于运送,安全有效的沙门氏菌疫苗。我们在这项研究中的目标是证明壳聚糖纳米颗粒(CNP)疫苗递送平台激活沙门氏菌免疫应答的能力口服肉鸡中的抗原。在最初的研究中,接种了被CNP包裹的SE的可溶性全抗原,但特异性免疫反应较差。因此,开发了CNP捕获的SE的免疫原性外膜蛋白(OMP)和鞭毛蛋白(FLA),并与FLA [CNP-(OMP + FLA)]共轭。在一次或两次口服CNP-(OMP + FLA)配方的肉鸡中,我们监测了先天免疫分子的时间表达和抗原特异性淋巴细胞的增殖。在接种CNP-(OMP + FLA)的盲肠扁桃体中,我们观察到编码Toll样受体(TLR)-1、4、5和7的mRNA表达增强,尤其是在dpv 21时。 CNP-(OMP + FLA)在dpv 7和21处的FLA特异性淋巴细胞增殖在脾脏中增强。结论,CNP-(OMP + FLA)制剂增强了口服接种肉鸡的先天和淋巴细胞应答。在挑战试验中,需要进一步的研究来确定候选亚单位CNP疫苗的功效。

更新日期:2020-08-23
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