Signaling pathways and transcription factors are involved in porcine embryonic development. Here, we demonstrate that glycogen synthase kinase-3 (GSK3) inhibitor, CHIR99021 and recombinant porcine interleukin-6 (rpIL6) significantly promote porcine parthenogenetic blastocyst formation (49.23 ± 8.40% vs 32.34 ± 4.15%), with increased inner cell mass (ICM) cell numbers (7.72 ± 2.30 vs 4.28 ± 1.60) and higher expression of pluripotent genes, such as OCT4, SOX2 and NANOG. Furthermore, CHIR99021 and rpIL6 improve blastocyst quality with increased blastocyst hatching percentage (16.19 ± 1.96% vs 10.25 ± 1.12%) and subsequently porcine pluripotent stem cells (pPSCs) derivation efficiency. These results advance the understanding of porcine pre-implantation development and provide evidences in improving the blastocyst quality.

中文翻译：

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CHIR99021和rpIL6促进猪孤雌生殖胚胎发育和囊胚质量

信号通路和转录因子参与猪胚胎发育。在这里，我们证明糖原合酶激酶 3 (GSK3) 抑制剂 CHIR99021 和重组猪白介素 6 (rpIL6) 显着促进猪孤雌生殖囊胚形成（49.23 ± 8.40% vs 32.34 ± 4.15%），并增加内细胞质量（ICM） ) 细胞数 (7.72 ± 2.30 vs 4.28 ± 1.60) 和多能基因的更高表达，例如 OCT4、SOX2 和 NANOG。此外，CHIR99021 和 rpIL6 提高了囊胚质量，提高了囊胚孵化率（16.19 ± 1.96% vs 10.25 ± 1.12%），随后提高了猪多能干细胞 (pPSCs) 的衍生效率。这些结果促进了对猪着床前发育的理解，并为提高囊胚质量提供了证据。