The aim of this work is to evaluate the chemical constituents and potential biological activists of Cunninghamella blakesleeana. Three fatty acids were isolated using column chromatography and identified as palmitic acid (F1), oleic acid (F2) and stearic acid (F3) in addition to other two steroidal compounds; α-amyrin (A4), and β-sitosterol (A5). Using GC, ten fatty acids were detected the major fatty acid obtained was stearic acid (74.61%) while palmitic acid was the second high percentage (10.35%), and the least percentage obtained was arachidic acid (0.07%). C. blakesleeana extract showed in-vitro antimicrobial activities against some microorganisms. The highest activity of C. blakesleeana total extract was reported against Staphylococcus aureus (18.3 ± 0.03 mm.) followed by Streptococcus pyogenes (15.3 ± 0.05), while the lowest were for both Candida albicans & Pseudomonas aeruginosa (6.7 ± 0.06 and 5.9.0 ± 0.9 mm. respectively). The three isolated compounds (F1-3) showed activities against Staphylococcus aureus, Penicillium expansum, and Salmonella typhimurium only. The highest activity was aganist Staphylococcus aureus (13.0 ± 0.1 mm.). The highest effect was obtained by compound F3 (stearic acid) (15.0 ± 0.5 mm.), and compound F1 (oleic acid) (13.0 ± 0.1 mm.) and F2 (palmitic acid) 11.0 ± 0.3 mm. The total ethanol extract of the investigated fungus was safe up to 5000 mg kg⁻¹ and did not produce any significant change in liver and kidney functions after oral administration (400 mg kg⁻¹) for 14 consecutive days. The results reported the isolation of some fungal new driving compounds which has been not isolated before from Cunninghamella species in addition to their correlated new biological activities.

这项工作的目的是评估Cunninghamella blakesleeana的化学成分和潜在的生物活性剂。使用柱色谱法分离了三种脂肪酸，除其他两种甾族化合物外，鉴定为棕榈酸（F1），油酸（F2）和硬脂酸（F3）；α-amyrin（A4）和β-谷甾醇（A5）。使用GC检测到十种脂肪酸，主要脂肪酸为硬脂酸（74.61％），而棕榈酸为第二高百分比（10.35％），最小百分比为花生酸（0.07％）。C. blakesleeana提取物对某些微生物表现出体外抗菌活性。活性最高C. blakesleeana总提取物对报道金黄色葡萄球菌（18.3±0.03 mm。），随后是化脓性链球菌（15.3±0.05），最低的是白色念珠菌和铜绿假单胞菌（分别为6.7±0.06和5.9.0±0.9 mm）。三种分离的化合物（F1-3）仅对金黄色葡萄球菌，扩展青霉和鼠伤寒沙门氏菌具有活性。活性最高的是根系金黄色葡萄球菌（13.0±0.1毫米）。通过化合物F3（硬脂酸）（15.0±0.5mm。），化合物F1（油酸）（13.0±0.1mm。）和F2（棕榈酸）11.0±0.3mm，可获得最高的效果。所研究真菌的总乙醇提取物安全至5000 mg kg^-1，并且连续14天口服（400 mg kg ^-1）后，肝肾功能没有任何明显变化。结果报道了一些真菌新的驱动化合物的分离，这些化合物除了与它们相关的新的生物活性外，还从未从坎宁氏菌中分离出来。