Abstract Here we reported an inner filter effect (IFE)-based simple assay system for the detection of glucose and hydrogen peroxide in the absence of glucose oxidase (GOx) enzyme, taking the advantage of the intrinsic GOx-like catalytic activity of gold nanoparticles (AuNPs). In this assay, the AuNPs were applied as GOx-mimicking catalysts to oxidation of glucose for in-situ production of H2O2, which in turn, can react with HAuCl4 to produce Au0 for deposition on the AuNPs core and result in the enlargement of self-catalyzed AuNPs and alteration of its absorption spectrum. The intensity of the surface Plasmon resonance (SPR) absorption of enlarged AuNPs was found to increase proportional to increasing concentration of H2O2 or glucose. Because of the relatively strong overlap between the SPR absorption of AuNPs (as absorber) and the fluorescence emission of histidine-protected gold nanoclusters (His-AuNCs) (as fluorophore), the fluorescence intensity of His-AuNCs decreased via IFE of AuNPs as the concentrations of H2O2 or glucose increased. This IFE based assay exhibited good performance towards the detection of hydrogen peroxide (over 5–135 μM) and glucose (over 5–125 μM) with detection limits of 3.6 μM and 3.4 μM for H2O2 and glucose, respectively. The method was finally applied to highly selective detection of glucose in urine sample of a diabetes patient.

中文翻译：

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基于金纳米粒子的葡萄糖氧化酶模拟特性，组氨酸封端的金纳米团簇介导葡萄糖和过氧化氢的荧光检测，通过内过滤效应机制

摘要 在这里，我们报道了一种基于内过滤效应 (IFE) 的简单测定系统，用于在没有葡萄糖氧化酶 (GOx) 酶的情况下检测葡萄糖和过氧化氢，利用金纳米粒子固有的类 GOx 催化活性。金纳米粒子）。在该试验中，AuNPs 被用作 GOx 模拟催化剂，用于葡萄糖的氧化以原位产生 H2O2，H2O2 又可以与 HAuCl4 反应产生 Au0 以沉积在 AuNPs 核心上，并导致自催化 AuNPs 并改变其吸收光谱。发现增大的 AuNP 的表面等离子体共振 (SPR) 吸收强度与 H2O2 或葡萄糖浓度的增加成正比。由于 AuNPs（作为吸收剂）的 SPR 吸收与组氨酸保护的金纳米团簇（His-AuNCs）（作为荧光团）的荧光发射之间存在相对较强的重叠，His-AuNCs 的荧光强度通过 AuNPs 的 IFE 降低H2O2 或葡萄糖的浓度增加。这种基于 IFE 的测定在检测过氧化氢（超过 5-135 μM）和葡萄糖（超过 5-125 μM）方面表现出良好的性能，对 H2O2 和葡萄糖的检测限分别为 3.6 μM 和 3.4 μM。该方法最终应用于糖尿病患者尿样中葡萄糖的高选择性检测。这种基于 IFE 的测定在检测过氧化氢（超过 5-135 μM）和葡萄糖（超过 5-125 μM）方面表现出良好的性能，对 H2O2 和葡萄糖的检测限分别为 3.6 μM 和 3.4 μM。该方法最终应用于糖尿病患者尿样中葡萄糖的高选择性检测。这种基于 IFE 的测定在检测过氧化氢（超过 5-135 μM）和葡萄糖（超过 5-125 μM）方面表现出良好的性能，对 H2O2 和葡萄糖的检测限分别为 3.6 μM 和 3.4 μM。该方法最终应用于糖尿病患者尿样中葡萄糖的高选择性检测。