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HPLC enantio-separation and chiral recognition mechanism of quinolones on vancomycin CSP.
Journal of Chromatography B ( IF 3 ) Pub Date : 2020-08-17 , DOI: 10.1016/j.jchromb.2020.122335
Zeid A ALOthman 1 , Abdullah G ALanazi 1 , Mohd Suhail 2 , Imran Ali 3
Affiliation  

A new HPLC method was developed for the enantio-separation and chiral recognition mechanism of quinolones (lomefloxacine, ofloxacine, primaquine and quinacrine) on Vancomycin CSP. The column used was Chirabiotic V column (150 × 4.6 mm, 5.0 μm) with two mobile phases i.e. (I) MeOH:ACN:H2O:TEA (50:30:20:0.1%) and (II) MeOH:ACN: H2O:TEA (70:10:20:0.1%) at 1.0 mL/minute flow rate with various UV detection. The values of retention, separation and resolution factors in a solvent system I were ranged from 2.20 to 5.05, 1.70 to 1.96 and 1.75 to 2.20 while these values were 1.93 to 6.85, 1.62 & 2.01 and 2.30 & 2.40 in solvent system II. The limits of detection and quantification were ranged from 8.0 to 10.5 µg and 24.4 to 33.5 µg. The resolution was controlled mainly by π-π interactions along with other forces like hydrogen bonding, van der Waal’s forces, steric effects, etc. The determination of the chiral recognition mechanism may be beneficial to separate other racemates successfully. The method is fast precise and efficient and may be utilized to analyze enantiomers of the reported quinolones.



中文翻译:

万古霉素CSP上喹诺酮的HPLC对映分离和手性识别机理。

开发了一种新的HPLC方法,用于在万古霉素CSP上对喹诺酮(洛美沙星,氧氟沙星,伯氨喹和奎纳克林)的对映分离和手性识别机理。使用的色谱柱是具有两个流动相的Chirabiotic V色谱柱(150×4.6 mm,5.0μm),即(I)MeOH:ACN:H 2 O:TEA(50:30:20:0.1%)和(II)MeOH:ACN :高2O:TEA(70:10:20:0.1%)以1.0 mL /分钟的流速进行各种UV检测。溶剂系统I中的保留,分离和拆分因子的值范围为2.20至5.05、1.70至1.96和1.75至2.20,而溶剂系统II中的保留值,分离度和分离度值为1.93至6.85、1.62&2.01和2.30&2.40。检测和定量的范围为8.0至10.5 µg和24.4至33.5 µg。分离度主要受π-π相互作用以及氢键,范德华力,空间效应等其他作用力的控制。手性识别机制的确定可能有利于成功分离其他外消旋物。该方法快速准确,高效,可用于分析已报道喹诺酮类的对映体。

更新日期:2020-08-29
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