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A live attenuated Edwardsiella tarda vaccine induces immunological expression pattern in Japanese flounder (Paralichthys olivaceus) in the early phase of immunization.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-08-16 , DOI: 10.1016/j.cbpc.2020.108872
Xue-Peng Li 1 , Jian Zhang 1
Affiliation  

A previous study showed that an attenuated Edwardsiella tarda strain, TXhfq, as a live vaccine could elicit protective immune effects in fish against E. tarda infection. In the current study, in order to clarify the molecular mechanism of fish immune response at the early stage after TXhfq vaccination, RNA-Seq technology was used to compare the transcriptomes of skin, intestine, and spleen between bath-vaccinated and unvaccinated Japanese flounder (Paralichthys olivaceus). An average of 46.6 million clean reads per library was obtained, ~88.04% of which were successfully mapped to the reference genome, and approximately 24,600 genes were detected in each sample. A total of 565, 878, and 1258 differential expression genes (DEGs) were found in skin, intestine, and spleen, respectively, including 1263 up-regulated genes and 1438 down-regulated genes. The DEGs exhibited different characteristics in each tissue. One hundred and sixteen DEGs belonging to six immune related categories were scrutinized, i.e., inflammatory factors, cytokines, complement and coagulation system, mucins, phagocytosis, and antigen processing and presentation. A protein-protein interaction network was constructed to get the interaction network between immune genes during the early stage of immunization. The top six hub genes highly regulated by TXhfq formed complicated interaction relationship with each other, which were involved in immune processes, notably inflammation and phagocytosis. Our results provide valuable information for the understanding of the immune mechanism underlying the protection of live attenuated vaccines in fish.



中文翻译:

减毒的爱德华氏菌活的减毒活疫苗在免疫初期可诱导日本比目鱼(Paralichthys olivaceus)的免疫表达方式。

先前的一项研究表明,减毒的爱德华氏菌缓释菌株TXhfq作为活疫苗可以在鱼类中引起针对泰大肠杆菌感染的保护性免疫作用。在当前的研究中,为了阐明TXhfq疫苗接种后早期鱼类免疫反应的分子机制,使用RNA-Seq技术比较了浴接种和未接种的日本比目鱼之间皮肤,肠和脾的转录组(橄榄草)。每个文库平均获得4660万个纯读,其中〜88.04%已成功定位到参考基因组,每个样品中检测到约24,600个基因。分别在皮肤,肠和脾中发现总共565、878和1258个差异表达基因(DEG),包括1263个上调基因和1438个下调基因。DEG在每个组织中表现出不同的特征。仔细检查了属于六个免疫相关类别的116个DEG,即炎症因子,细胞因子,补体和凝血系统,粘蛋白,吞噬作用以及抗原加工和呈递。构建蛋白质-蛋白质相互作用网络以在免疫的早期获得免疫基因之间的相互作用网络。受TXhfq高度调节的前6个枢纽基因彼此之间形成复杂的相互作用关系,参与免疫过程,尤其是炎症和吞噬作用。我们的结果为了解保护鱼类减毒活疫苗背后的免疫机制提供了有价值的信息。

更新日期:2020-08-28
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