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Bio‐probing with nonresonant surface‐enhanced hyper‐Raman scattering excited at 1,550 nm
Journal of Raman Spectroscopy ( IF 2.5 ) Pub Date : 2020-08-16 , DOI: 10.1002/jrs.5965
Zsuzsanna Heiner 1, 2 , Fani Madzharova 1 , Vesna Živanović 1, 2 , Janina Kneipp 1, 2
Affiliation  

The two‐photon excited process of surface‐enhanced hyper‐Raman scattering (SEHRS) provides advantages for studies of complex biological samples, yet suitable SEHRS nanoprobes and labels, as well as experimental conditions, must be established. Here, SEHRS spectra of the four reporter molecules (2‐naphthalenethiol [2‐NAT], para‐aminothiophenol (pATP), para‐nitrothiophenol (pNTP), and crystal violet), as well as the two antidepressant drugs (desipramine and imipramine) were obtained at an excitation wavelength of 1,550 nm using different citrate‐stabilized gold nanoparticles and silver nanoparticles, and under conditions that permit experiments with living cultured cells. As the results suggest, the short‐wave infrared laser excitation and the hyper‐Raman scattered light (corresponding to wavelengths > 775 nm) match the plasmonic properties of the employed gold and silver nanoaggregates, as well as the requirements regarding the viability of the cells. The two‐photon excited spectra of three types of SEHRS labels containing 2‐NAT as reporter inside macrophage cells show that molecules in the cellular environment can also be observed. The possibility to use short‐wave infrared excitation with gold nanostructures has important implications for the utilization of SEHRS in bio‐probing.

中文翻译:

在1,550 nm激发的非共振表面增强超拉曼散射进行生物探测

表面增强的超拉曼散射(SEHRS)的双光子激发过程为研究复杂的生物样品提供了优势,但必须建立合适的SEHRS纳米探针和标记物以及实验条件。在这里,四个报告分子(2-萘硫醇[2-NAT],氨基硫酚(p ATP),硝基硫酚(p(NTP)和结晶紫)以及两种抗抑郁药(desipramine和imipramine)在1,550 nm的激发波长下使用不同的柠檬酸盐稳定的金纳米颗粒和银纳米颗粒,并在允许进行活细胞培养的条件下获得。结果表明,短波红外激光激发和超拉曼散射光(对应于波长> 775 nm)符合所用金和银纳米聚集体的等离子体性能,以及对细胞活力的要求。巨噬细胞内部三种含有2-NAT作为报告基因的SEHRS标签的双光子激发光谱表明,还可以观察到细胞环境中的分子。
更新日期:2020-08-16
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