Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple bZIP s have been identified and investigated in numerous plant species. However, few studies have characterized bZIP s from Dimocarpus longan Lour. In this study, nine bZIP s from D. longan were identified from RNA-Seq data and further verified using the NCBI conserved domain search tool and Pfam database. Bioinformatics tools were used to systematically analyse the physicochemical properties, protein structures, multiple sequence alignment, motif compositions, evolutionary relationships, secondary structures, subcellular localization, phosphorylation sites, signal peptides, GO annotations and protein–protein interactions of the DlbZIP s. The expression patterns of the nine DlbZIP s were evaluated by qRT-PCR in roots and leaves and in response to varying durations of a 38°C heat treatment. DlbZIP3 , DlbZIP5 , DlbZIP6 and DlbZIP7 were differentially expressed between root and leaf tissues. All nine DlbZIPs responded to heat treatment in both roots and leaves, but their specific expression levels differed. DlbZIP4 and DlbZIP8 were highly expressed in roots after heat treatment, whereas DlbZIP1 and DlbZIP5 were highly expressed in leaves after heat treatment. These findings lay a foundation for increasing active secondary metabolite content and improving abiotic stress tolerance in D. longan using transgenic technology.

中文翻译：

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龙眼龙眼 bZIP 转录因子的表征。并分析它们的组织特异性表达模式和对热应激的反应

bZIP 转录因子家族的成员在调节植物发育、次生代谢物的生物合成以及对非生物和生物胁迫的响应中起着至关重要的作用。迄今为止，已在许多植物物种中鉴定并研究了多个 bZIP。然而，很少有研究对来自龙眼龙眼的 bZIP 进行表征。在这项研究中，九个来自龙眼的 bZIP 从 RNA-Seq 数据中被识别出来，并使用 NCBI 保守域搜索工具和 Pfam 数据库进一步验证。使用生物信息学工具系统地分析了 DlbZIP 的理化特性、蛋白质结构、多序列比对、基序组成、进化关系、二级结构、亚细胞定位、磷酸化位点、信号肽、GO 注释和蛋白质 - 蛋白质相互作用。九个 DlbZIP 的表达模式通过 qRT-PCR 在根和叶中进行评估，并响应于 38°C 热处理的不同持续时间。DlbZIP3、DlbZIP5、DlbZIP6和DlbZIP7在根和叶组织之间差异表达。所有九个 DlbZIP 在根和叶中都对热处理有反应，但它们的特定表达水平不同。DlbZIP4 和 DlbZIP8 在热处理后在根中高表达，而 DlbZIP1 和 DlbZIP5 在热处理后在叶片中高表达。这些发现为利用转基因技术提高龙眼活性次生代谢产物含量和提高非生物胁迫耐受性奠定了基础。DlbZIP5、DlbZIP6和DlbZIP7在根和叶组织之间差异表达。所有九个 DlbZIP 在根和叶中都对热处理有反应，但它们的特定表达水平不同。DlbZIP4和DlbZIP8在热处理后在根中高表达，而DlbZIP1和DlbZIP5在热处理后在叶中高表达。这些发现为利用转基因技术提高龙眼活性次生代谢产物含量和提高非生物胁迫耐受性奠定了基础。DlbZIP5、DlbZIP6和DlbZIP7在根和叶组织之间差异表达。所有九个 DlbZIP 在根和叶中都对热处理有反应，但它们的特定表达水平不同。DlbZIP4 和 DlbZIP8 在热处理后在根中高表达，而 DlbZIP1 和 DlbZIP5 在热处理后在叶片中高表达。这些发现为利用转基因技术提高龙眼活性次生代谢产物含量和提高非生物胁迫耐受性奠定了基础。而 DlbZIP1 和 DlbZIP5 在热处理后的叶子中高度表达。这些发现为利用转基因技术提高龙眼活性次生代谢产物含量和提高非生物胁迫耐受性奠定了基础。而 DlbZIP1 和 DlbZIP5 在热处理后的叶子中高度表达。这些发现为利用转基因技术提高龙眼活性次生代谢产物含量和提高非生物胁迫耐受性奠定了基础。