Genome-wide insights of Ethiopian indigenous sheep populations reveal the population structure related to tail morphology and phylogeography.,Genes & Genomics

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Genome-wide insights of Ethiopian indigenous sheep populations reveal the population structure related to tail morphology and phylogeography.
Genes & Genomics ( IF 2.1 ) Pub Date : 2020-08-16 , DOI: 10.1007/s13258-020-00984-y
Agraw Amane _{1,

2,

3,

4} , Gurja Belay ₁ , Yao Nasser ₃ , Martina Kyalo ₃ , Tadelle Dessie ₄ , Adebabay Kebede ₂ , Tesfaye Getachew ₅ , Jean-Baka Domelevo Entfellner ₃ , Zewdu Edea ₆ , Olivier Hanotte _{4,

7} , Getinet Mekuriaw Tarekegn _{8,

9}

Affiliation

Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia.
Andassa Livestock Research Center, Amhara Regional Agricultural Research Institute, Bahir Dar, Ethiopia.
Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya.
LiveGene Program, International Livestock Research Institute, Addis Ababa, Ethiopia.
International Center for Agricultural Research in the Dry Areas, Addis Ababa, Ethiopia.
Department of Animal Science, Chungbuk National University, Cheongju, South Korea.
School of Life Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Department of Animal Breeding and Genetics, Swedish University of Agricultural Science (SLU), Uppsala, Sweden.
Department of Animal Production and Technology, School of Animal Sciences and Veterinary Medicine, Bahir Dar University, Bahir Dar, Ethiopia.

Background

Ethiopian sheep living in different climatic zones and having contrasting morphologies are a most promising subject of molecular-genetic research. Elucidating their genetic diversity and genetic structure is critical for designing appropriate breeding and conservation strategies.

Objective

The study was aimed to investigate genome-wide genetic diversity and population structure of eight Ethiopian sheep populations.

Methods

A total of 115 blood samples were collected from four Ethiopian sheep populations that include Washera, Farta and Wollo (short fat-tailed) and Horro (long fat-tailed). DNA was extracted using Quick-DNA™ Miniprep plus kit. All DNA samples were genotyped using Ovine 50 K SNP BeadChip. To infer genetic relationships of Ethiopian sheep at national, continental and global levels, genotype data on four Ethiopian sheep (Adilo, Arsi-Bale, Menz and Black Head Somali) and sheep from east, north, and south Africa, Middle East and Asia were included in the study as reference.

Results

Mean genetic diversity of Ethiopian sheep populations ranged from 0.352 ± 0.14 for Horro to 0.379 ± 0.14 for Arsi-Bale sheep. Population structure and principal component analyses of the eight Ethiopian indigenous sheep revealed four distinct genetic cluster groups according to their tail phenotype and geographical distribution. The short fat-tailed sheep did not represent one genetic cluster group. Ethiopian fat-rump sheep share a common genetic background with the Kenyan fat-tailed sheep.

Conclusion

The results of the present study revealed the principal component and population structure follows a clear pattern of tail morphology and phylogeography. There is clear signature of admixture among the study Ethiopian sheep populations

中文翻译：

埃塞俄比亚本土绵羊种群的全基因组洞察揭示了与尾部形态和系统地理学相关的种群结构。

背景

埃塞俄比亚绵羊生活在不同的气候带并具有不同的形态，是分子遗传研究中最有前途的课题。阐明它们的遗传多样性和遗传结构对于设计适当的育种和保护策略至关重要。

客观的

该研究旨在调查八个埃塞俄比亚绵羊种群的全基因组遗传多样性和种群结构。

方法

从四个埃塞俄比亚绵羊种群中收集了总共 115 份血液样本，包括 Washera、Farta 和 Wollo（短肥尾）和 Horro（长肥尾）。使用 Quick-DNA™ Miniprep plus 试剂盒提取 DNA。使用 Ovine 50 K SNP BeadChip 对所有 DNA 样品进行基因分型。为了在国家、大陆和全球层面推断埃塞俄比亚绵羊的遗传关系，四种埃塞俄比亚绵羊（Adilo、Arsi-Bale、Menz 和 Black Head Somali）以及来自东非、北非、南非、中东和亚洲的绵羊的基因型数据进行了分析。纳入研究作为参考。

结果

埃塞俄比亚绵羊种群的平均遗传多样性范围从 Horro 的 0.352 ± 0.14 到 Arsi-Bale 绵羊的 0.379 ± 0.14。八只埃塞俄比亚土著绵羊的种群结构和主成分分析揭示了四个不同的遗传簇群，根据它们的尾巴表型和地理分布。短肥尾羊不代表一个遗传簇群。埃塞俄比亚肥臀羊与肯尼亚肥尾羊有着共同的遗传背景。