当前位置: X-MOL 学术Ann. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bacterial laccase of Anoxybacillus ayderensis SK3-4 from hot springs showing potential for industrial dye decolorization
Annals of Microbiology ( IF 3 ) Pub Date : 2020-08-14 , DOI: 10.1186/s13213-020-01593-6
Jingjing Wang , Fei Chang , Xiaoqing Tang , Wei Li , Qiang Yin , Yang Yang , Yang Hu

Laccases are green biocatalysts that possess attractive for the treatment of resistant environmental pollutants and dye effluents. To exploit the laccase of Anoxybacillus ayderensis SK3-4 that possesses dye decolorization ability at room and higher temperature, we characterized the enzyme in considerable detail and investigated its ability to decolorize different dyes. A bacterial laccase gene designed as LacAn from Anoxybacillus ayderensis SK3-4 of hot springs was cloned and expressed in Escherichia coli. LacAn is a monomeric protein with a molecular weight of 29.8 kDa. The optimum pH and temperature for syringaldazine oxidation were 7.0 and 75 °C, respectively. LacAn was stable at pH values ranging from 6.5 to 8.5 above 65 °C. The enzyme activity was significantly enhanced by Cu2+ and Mg2+ but inhibited by Zn2+ and Fe2+. Furthermore, LacAn showed high decolorization capability toward five dyes (direct blue 6, acid black 1, direct green 6, direct black 19, and acid blue 93) in the absence of redox mediators. It also demonstrated a wide temperature range, and it can retain its high decolorization ability even at high temperatures. These properties including better enzymatic properties and efficiency to decolorize dyes demonstrate that the bacterial laccase LacAn has potentials for further industrial applications.

中文翻译:

来自温泉的Ayderbacillus ayderensis SK3-4细菌漆酶显示出工业染料脱色的潜力

漆酶是绿色的生物催化剂,对治疗抗性环境污染物和染料废水具有吸引力。为了利用在室温和更高温度下具有染料脱色能力的无性漆酶SK3-4漆酶,我们对酶进行了相当详细的表征,并研究了其使不同染料脱色的能力。克隆了一种从温泉无性系Ayderbacensis Ayderensis SK3-4设计为LacAn的细菌漆酶基因,并在大肠杆菌中表达。LacAn是一种单体蛋白,分子量为29.8 kDa。丁香醛嗪氧化的最佳pH和温度分别为7.0和75°C。LacAn在65°C以上的pH值范围为6.5至8.5时稳定。酶活性被Cu2 +和Mg2 +显着增强,但被Zn2 +和Fe2 +抑制。此外,在不存在氧化还原介体的情况下,LacAn对五种染料(直接蓝6,酸性黑1,直接绿6,直接黑19和酸性蓝93)具有很高的脱色能力。它还显示了宽温度范围,即使在高温下也可以保持其高脱色能力。这些特性包括更好的酶促特性和使染料脱色的效率,证明细菌漆酶LacAn具有进一步工业应用的潜力。
更新日期:2020-08-15
down
wechat
bug