Amorphophallus

has attracted tremendous interest because of its high contents of glucomannan and starch. Very few genes regulating glucomannan and starch were reported in Amorphophallus. In this study, an ADP-glucose pyrophosphorylase (AGP) gene that plays a significant role in plant starch synthesis was cloned from Amorphophallus muelleri. It was shown that it encoded a predicted protein containing a conserved plant ADP-Glucose-PP repeat domain and seven potential ligand-binding sites. The real-time quantitative PCR showed that AmAGP was most abundant in tubers, and it was positively correlated with starch content. Additionally, its influencers about temperature and exogenous plant hormone were also discussed, showing that AmAGP expressed highly in tubers under treatments using 25°C and IAA. Furthermore, starch content was closely related to AmAGP expression level, suggesting that AmAGP was involved in the regulation of starch synthesis in A. muelleri. Therefore, identifying the sequence of AmAGP and its expression pattern during tuber enlarging and the changes of its transcript levels in response to temperature and plant hormones would contribute to a better understanding of starch synthesis, and also providing a reference information for future preferable breeding for obtaining more starch or more glucomannan in Amorphophallus.

中文翻译：

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魔芋中淀粉合成相关基因AmAGP的功能表征

魔芋

葡甘露聚糖和淀粉含量高，因此引起了极大的兴趣。在魔芋中报道很少调控葡甘露聚糖和淀粉的基因。在这项研究中，从Amorphophallus muelleri克隆了在植物淀粉合成中起重要作用的ADP-葡萄糖焦磷酸化酶（AGP）基因。结果表明，它编码一种预测的蛋白质，该蛋白质包含一个保守的植物ADP-葡萄糖-PP重复结构域和七个潜在的配体结合位点。实时定量PCR表明，AmAGP在块茎中含量最高，且与淀粉含量呈正相关。此外，还讨论了其对温度和外源植物激素的影响，表明AmAGP在使用25°C和IAA处理的块茎中高表达。此外，淀粉含量密切相关AmAGP表达水平，这表明AmAGP参与淀粉合成的调节A. muelleri。因此，鉴定块茎膨大过程中AmAGP的序列及其表达方式，以及其转录水平随温度和植物激素的变化，将有助于人们更好地了解淀粉的合成，并为以后的优选育种提供参考信息。魔芋中含有更多的淀粉或葡糖甘露聚糖。