Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details to perform heterotopic and partial orthotopic surgical models to promote ALS transplantation. After preservation and vessel cannulation steps, the liver grafts were decellularized. In addition, ex vivo blood perfusion tests were performed to obtain a successful anticoagulation treatment prior in vivo transplantation. Then, methods of partial liver resection, combination of hand-suture and cuff techniques to complete end-to-end anastomosis between the scaffold and the recipient animal were performed. These procedures which take 30–60 min and were efficient to allow acellular liver scaffold viability and recellularization of different types of cell post-surgery. In conclusion, our methods are practical and simple promising approach that provides the opportunity to investigate ways to achieve sufficient liver function post-transplantation in vivo.

无细胞肝支架（ALS）已成为潜在的移植候选对象。到目前为止，所有涉及ALS移植的报道均未能通过手术方法进行描述，并且并未为科学家提供支持以重现用于实验显微外科手术的程序，以使结果可与文献相提并论。为了克服详细信息的缺乏，我们描述了外科手术步骤的详细信息，以执行异位和部分原位手术模型来促进ALS移植。保存并进行血管插管步骤后，将肝脏移植物脱细胞。此外，进行了体外血液灌注测试，以在体内之前获得成功的抗凝治疗移植。然后，进行了部分肝切除的方法，手工缝合和袖套技术的结合，以完成支架与受者动物之间的端到端吻合。这些程序耗时30-60分钟，有效地使无细胞肝支架存活并在术后进行不同类型的细胞再细胞化。总之，我们的方法是实用且简单的有前途的方法，为研究体内移植后实现足够肝功能的方法提供了机会。