Herein, we investigated the effect of electrospun polycaprolactone fumarate (PCLF) nanofibers on neural stem cell (NSC) behavior in the in vitro setting. Murine NSCs were isolated from adult mice subventricular zone and immunophenotyped by flow cytometry assay and immunofluorescence staining. Cells were cultured on the plastic surface, laminin‐coated surface, and electrospun PCLF nanofibers. Cell morphology, attachment, and spreading were evaluated by scanning electron microscopy analysis. Cell viability and proliferation rates were evaluated by MTT assay. The proliferation of plated cells was investigated by monitoring Ki‐67‐positive cells using flow cytometry analysis. The protein levels of Map‐2 and GFAP were detected by using immunofluorescence staining to show neural and astrocyte differentiation capacity. Scanning electron microscopy images revealed an extensive distribution, morphological adaptation, and cell‐to‐cell connectivity in NSCs upon culture on the PCLF surface. MTT analysis showed that the NSCs had more survival rates on the PCLF surface compared to the laminin and control groups over time (p < 0.05). In contrast to the laminin group, Ki‐67 analysis showed a decrease of proliferating cells in the PCLF group. Immunofluorescence staining revealed the prominent increase of Map‐2 and GFAP reduction in NSCs from the PCLF group compared to the laminin and control groups, showing the stimulatory effect of PCLF on targeted maturation of NSCs (p < 0.05). In brief, PCLF based construct promotes NSCs morphological adaptation and neuronal differentiation, suggesting PCLF as an appropriate and applicable substrate in neural tissue engineering.

中文翻译：

---

聚己内酯富马酸酯作为人工神经网络促进神经干细胞的生物学行为。

在此，我们研究了电纺聚己内酯富马酸酯 (PCLF) 纳米纤维对体外环境中神经干细胞 (NSC) 行为的影响。从成年小鼠脑室下区分离小鼠神经干细胞，并通过流式细胞术测定和免疫荧光染色进行免疫表型分析。在塑料表面、层粘连蛋白涂层表面和电纺 PCLF 纳米纤维上培养细胞。通过扫描电子显微镜分析评估细胞形态、附着和扩散。通过MTT测定评估细胞活力和增殖率。通过使用流式细胞术分析监测 Ki-67 阳性细胞来研究铺板细胞的增殖。使用免疫荧光染色检测 Map-2 和 GFAP 的蛋白质水平，以显示神经和星形胶质细胞的分化能力。扫描电子显微镜图像显示，在 PCLF 表面培养后，NSCs 广泛分布、形态适应和细胞间连接。MTT 分析表明，随着时间的推移，与层粘连蛋白组和对照组相比，NSCs 在 PCLF 表面的存活率更高。p < 0.05)。与层粘连蛋白组相比，Ki-67 分析显示 PCLF 组中增殖细胞减少。免疫荧光染色显示，与层粘连蛋白组和对照组相比，PCLF 组 NSCs 的 Map-2 和 GFAP 减少显着增加，表明 PCLF 对 NSCs 靶向成熟的刺激作用（p < 0.05）。简而言之，基于 PCLF 的构建体促进 NSC 的形态适应和神经元分化，表明 PCLF 是神经组织工程中合适且适用的底物。