Anaerobic digestion (AD) is a bioprocess for extracting renewable bio-methane fuel from a variety of biomass substrates. A growing body of literature indicate that the structure of the microbiota community closely correlates with methane yield during AD. The correlations between methane yield and the relative abundance (RA) of microbial community, however, is mainly qualitative. The goal of this research was to collate the published data to determine the quantitative nature and extent of such correlations. Available literature on AD providing methane yields and microbiota RA, suitable for the random model, were gathered from the web of sciences and other available data bases for the meta-analyses and meta-regressions. In total, 1140-case studies, comprising 846 mesophilic and 246 thermophilic-AD processes, were used in the analysis. Data heterogeneity indicated that the RA of microbiota against methane yield of AD were different at different operating conditions. The main bacteria significantly determining methane yield, during AD processes, were Firmicutes and Bacteroidetes. A unit increase (%) in the RA of Firmicutes resulted in about 2.9 mL g⁻¹[VS] improvement in CH₄ yield in non-stable mesophilic digesters, while the same improved CH₄ yield by 4.5 mL g⁻¹[VS] in stable mesophilic digesters. In stable mesophilic AD, a unit increase (%) in the RA of Bacteroidetes significantly improved CH₄ yield by 7.0 mL g⁻¹[VS] and by roughly 4.8 mL g⁻¹[VS] in non-stable mesophilic digesters. Significant decline in CH₄ yields corresponded to increases in the RA of two bacteria phyla (Actinobacteria and Proteobacteria) commonly related to overloaded digesters. In this regard, a unit increase (%) in Actinobacteria indicated a significant reduction in CH₄ yield (18.7 mL g⁻¹[VS]) in non-stable mesophilic digesters, while a unit increase (%) in Proteobacteria indicated around 4.9 mL g⁻¹[VS] decline in CH₄ yield in stable mesophilic digesters. The major archaea significantly contributing to CH₄ yield were Methanosarcina indicating CH₄ yields improvements ranging from 2.0 to 3.0 mL g⁻¹[VS] for each percent increase in the RA of Methanosarcina, in either mesophilic or thermophilic digesters. The RA of other minor microbiota groups did not statistically correlate with CH₄ yield. The HRT and OLR tended to positively correlate with the RA of Firmicutes, Bacteroidetes, and Methanosarcina.

厌氧消化（AD）是一种从多种生物质底物中提取可再生生物甲烷燃料的生物过程。越来越多的文献表明，AD期间微生物群落的结构与甲烷产量密切相关。但是，甲烷产量与微生物群落的相对丰度（RA）之间的相关性主要是定性的。这项研究的目的是整理已发布的数据，以确定这种相关性的定量性质和程度。适用于随机模型的有关提供甲烷产量和微生物群落RA的AD的现有文献是从科学网络和其他可用的数据库中收集的，用于荟萃分析和荟萃分析。在分析中，总共使用了1140个案例研究，包括846个嗜温过程和246个嗜热性AD过程。数据异质性表明，在不同的操作条件下，微生物群对AD甲烷产量的RA不同。在AD过程中，主要决定甲烷产量的主要细菌是硬菌和拟杆菌属。在不稳定的中温消化池中，Firmicutes RA的单位增加（％）导致CH ₄产量提高约2.9 mL g ^-1 [VS] ，而同样提高了4.5 mL g ^-1 [VS]的CH ₄产量在稳定的中温消化池中。在稳定的嗜温性AD中，拟杆菌的RA单位增加（％）可显着提高CH ₄产量，分别为7.0 mL g ^-1 [VS]和约4.8 mL g ^-1 [VS]。CH ₄产量的显着下降对应于两种细菌门的RA的增加（放线菌和变形杆菌通常与消化器超负荷有关。在这方面，放线菌单位增加（％）表明不稳定的中温消化池中CH ₄产量（18.7 mL g ^-1 [VS]）显着降低，而变形杆菌单位增加（％）约为4.9 mL在稳定的中温消化池中，CH ₄产量g ^-1 [VS]下降。对甲烷₄产生显着贡献的主要古细菌是甲烷八叠球菌，表明甲烷₄ RA的每增加百分数，甲烷_4的产量提高了2.0到3.0毫升g ^-1 [VS]。嗜甲烷菌，在嗜温或高温消化器中。其他次要微生物群的RA与CH ₄产量无统​​计学相关性。HRT和OLR倾向于与Firmicutes，Bacteroidetes和Methanosarcina的RA呈正相关。