Equine chorionic gonadotrophin (eCG) is a hormone widely used in superovulation protocols because of its follicle-stimulating action, which increases reproductive efficiency in animals of productive interest. It contains 45% carbohydrate, 10% of which is N-acetylneuraminic acid (sialic acid). The eCG purification procedures from equine serum or plasma are mainly based on chromatographic methods. However, before these procedures, it is necessary to follow sample pre-conditioning steps, such as several precipitation stages and/or ultrafiltration/diafiltration processes. In this work, an efficient affinity chromatographic matrix for eCG purification directly from plasma was developed. The matrix consisted of chitosan mini-spheres with immobilized wheat germ agglutinin (WGA). The matrix allowed 98% adsorption of eCG directly from plasma without any pre-treatment with an overall yield of around 60%. The matrix chosen was able to maintain the efficient performance of the purification process for three consecutive cycles. Also, the process was scaled-up 500 times in volume and tested over seven consecutive cycles maintaining its chromatographic performance. The results presented here suggest the potential application of this matrix to one-step purification of eCG from plasma.

马绒毛膜促性腺激素 (eCG) 是一种广泛用于超排卵方案的激素，因为它具有刺激卵泡的作用，可提高具有生产意义的动物的繁殖效率。它含有 45% 的碳水化合物，其中 10% 是 N-乙酰神经氨酸（唾液酸）。从马血清或血浆中纯化心电图的程序主要基于色谱法。然而，在这些程序之前，有必要遵循样品预处理步骤，例如几个沉淀阶段和/或超滤/渗滤过程。在这项工作中，开发了一种用于直接从血浆中纯化心电图的高效亲和色谱基质。基质由壳聚糖微球和固定化小麦胚芽凝集素 (WGA) 组成。该基质允许直接从血浆中吸附 98% 的心电图，无需任何预处理，总收率约为 60%。所选择的基质能够在连续三个循环中保持纯化过程的高效性能。此外，该过程的体积扩大了 500 倍，并在连续七个循环中进行了测试，以保持其色谱性能。此处显示的结果表明该基质在从血浆中一步纯化 eCG 中的潜在应用。