Recent studies have shown the relevance of growing mesenchymal stem cells (MSCs) in three-dimensional environments with respect to the monolayer cell culture on an adherent substrate. In this sense, macroporous scaffolds and hydrogels have been used as three-dimensional (3D) supports. In this work, we explored the culture of MSCs in a 3D environment created by microspheres, prepared with a fumarate-vinyl acetate copolymer and chitosan. In this system, the environment that the cells feel has similarities to that found by the cells encapsulated in a hydrogel, but the cells have the ability to reorganize their environment since the microspheres are mobile. We evaluated their biocompatibility in vitro using RAW 264.7 macrophages and bone marrow mesenchymal stem cells (BMSCs). The results with RAW 264.7 cells showed good cell viability, without evident signs of cytotoxicity. BMSCs not only proliferate, but also rearrange to grow in clusters, thus highlighting the advantages of microspheres as 3D environments.

最近的研究表明，在三维环境中生长的间充质干细胞（MSC）与在粘附基质上的单层细胞培养物的相关性。从这个意义上讲，大孔支架和水凝胶已被用作三维（3D）支撑。在这项工作中，我们探索了由富马酸酯-乙酸乙烯酯共聚物和壳聚糖制备的微球在3D环境下培养的MSCs的培养。在该系统中，细胞感觉的环境与封装在水凝胶中的细胞所具有的环境相似，但是由于微球是可移动的，因此细胞具有重组其环境的能力。我们在体外评估了它们的生物相容性使用RAW 264.7巨噬细胞和骨髓间充质干细胞（BMSC）。RAW 264.7细胞的结果显示出良好的细胞活力，没有明显的细胞毒性迹象。BMSC不仅增殖，而且重新排列成簇生长，因此突出了微球作为3D环境的优势。