Effective decontamination procedures are critical to the successful manufacture and control of poliovirus vaccines to minimize the risk to personnel and the environment. Polio viruses have been reported to be more resistant to disinfectants than many other viruses. We assessed the efficacy of sodium hypochlorite-containing disinfectants for decontamination for three poliovirus serotypes to implement decontamination procedures that are fully compliant with the WHO GAP III and Health authorities’ requirements. A 10.4 log reduction was observed with a 0.63% sodium hypochlorite solution in a suspension with high protein and high poliovirus concentrations diluted 10-fold compared with a 6 log reduction in an undiluted sample. Treatment efficacy increased with sodium hypochlorite content and decreased with sample protein content. The surface tests showed that two 1-min treatments, 5-min apart, with a 0.63% Chl sodium hypochlorite solution effectively reduced the concentration of all poliovirus serotypes by 10 log₁₀, irrespective of the protein and virus concentration in the sample. Sodium hypochlorite solutions lower than 0.52% were less effective for complete inactivation of poliovirus. In conclusion, we demonstrated that a high level of virus reduction (>10 log₁₀) can be achieved with sodium hypochlorite solutions with poliovirus in suspension and dried on surfaces.

有效的净化程序对于成功制造和控制脊灰病毒疫苗以最大限度地减少对人员和环境的风险至关重要。据报道，脊髓灰质炎病毒比许多其他病毒对消毒剂的抵抗力更强。我们评估了含有次氯酸钠的消毒剂对三种脊髓灰质炎病毒血清型的去污效果，以实施完全符合 WHO GAP III 和卫生当局要求的去污程序。与未稀释样品中的 6 log 降低相比，使用 0.63% 次氯酸钠溶液的高蛋白质和高脊灰病毒浓度稀释 10 倍的悬浮液观察到 10.4 log 降低。处理效果随次氯酸钠含量增加而增加，随样品蛋白质含量增加而降低。₁₀，不考虑样品中的蛋白质和病毒浓度。低于 0.52% 的次氯酸钠溶液对脊髓灰质炎病毒的完全灭活效果较差。总之，我们证明了使用悬浮脊灰病毒并在表面干燥的次氯酸钠溶液可以实现高水平的病毒减少 (>10 log ₁₀ )。