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Benzocaine and menthol as anesthetics for the African cichlid Aulonocara nyassae
Aquaculture International ( IF 2.9 ) Pub Date : 2020-05-21 , DOI: 10.1007/s10499-020-00561-w
Andre Lima Ferreira , Walisson de Souza e Silva , Luanna do Carmo Neves , Nathália Soares Ferreira , Rodrigo Takata , Ronald Kennedy Luz

The objective of this study was to investigate the effects of benzocaine and menthol on the anesthesia of Aulonocara nyassae juveniles. Two size classes of fish were used in trials with benzocaine: Juveniles I—70 fish of 0.74 ± 0.31 g (39.41 ± 7.48 mm); and Juveniles II—70 fish of 3.80 ± 0.92 g (76.58 ± 9.83 mm). The fish used for trials with menthol were as follows: Juveniles I—70 fish of 1.01 ± 0.39 g (50.39 ± 12.75 mm) and Juveniles II—70 fish of 3.73 ± 0.78 g (64.94 ± 8.98 mm). Seven concentrations—0, 12.5, 25.0, 50.0, 75.0, 100.0, and 125.0 mg L−1—of each anesthetic (benzocaine and menthol) were tested on each size class of fish (n = 10 fish per size class and anesthetic concentration). Thus, anesthesia induction time and recovery were evaluated. Concentrations of 12.5 and 25 mg L−1 of benzocaine did not lead to a deep stage of anesthesia in the animals; however, the other concentrations presented anesthetic effect. For menthol, the concentration of 12.5 mg L−1 also did not anesthetize the fish. However, the other doses were effective and safe. The recommended anesthetic concentrations provided induction and recovery times within the limits considered ideal for fish. Concentrations between 75 and 125 mg L−1 of benzocaine for Juvenile I and 50 to 125 mg L−1 for Juvenile II are recommended. For menthol, concentrations between 50 and 125 mg L−1 can be used for both size classes of A. nyassae.



中文翻译:

苯佐卡因和薄荷醇作为非洲丽鱼科丽鱼Aulonocara nyassae的麻醉剂

这项研究的目的是调查苯佐卡因和薄荷醇对Aulonocara nyassae少年麻醉的影响。在苯佐卡因试验中使用了两种规格的鱼:0.74±0.31克(39.41±7.48毫米)的I-70少年鱼;和II-70型幼鱼,3.80±0.92克(76.58±9.83毫米)。进行薄荷醇试验的鱼如下:I-70幼鱼1.01±0.39克(50.39±12.75毫米)和II-70幼鱼3.73±0.78克(64.94±8.98毫米)。在每种规格鱼上,对每种麻醉剂(苯佐卡因和薄荷醇)的七个浓度(0、12.5、25.0、50.0、75.0、100.0和125.0 mg L -1)进行了测试(n =每规格等级和麻醉浓度10条鱼)。因此,评估了麻醉诱导时间和恢复。苯并卡因的浓度为12.5和25 mg L -1不会导致动物进入较深的麻醉阶段。然而,其他浓度均表现出麻醉作用。对于薄荷醇,12.5 mg L -1的浓度也不会使鱼麻醉。但是,其他剂量是有效和安全的。推荐的麻醉剂浓度可使诱导和恢复时间均在鱼类理想的极限范围内。75和125毫克的L之间的浓度-1苯佐卡因少年I和50〜125毫克的L -1少年II建议。对于薄荷醇,浓度在50至125 mg L -1之间可以用于A. nyassae的两个大小级别。

更新日期:2020-05-21
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