Background Acetyl octapeptide-3 (SNAP-8) is an antiaging peptide that is more effective than acetyl hexapeptide-3, which is more stable than botulinum toxin and effectively relieves facial wrinkles. Products containing SNAP-8 such as patch are producing, but analytical method has not been reported to determination of SNAP-8. Method Mobile phase, collision energy, and desolvation line temperature were optimized, and mass spectral data set for SNAP-8 was newly constructed by liquid chromatography-triple quadrupole mass spectrometer in multiple reaction monitoring mode. Results The developed method showed good linearity ( r ≥ 0.9971) with limit of quantification of 0.0125 ng/mL, repeatability (% relative standard deviation = 0.02 to 0.12) and accuracy (% relative error = − 1.68 to 1.44) under optimal conditions. This method was successfully applied to a biodegradable microneedle patch loaded with SNAP-8. Conclusion The present method for the quantification of SNAP-8 may be useful for quality control in the cosmetic fields.

中文翻译：

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液相色谱-串联质谱法分析乙酰八肽 3 的方法开发

背景乙酰八肽-3（SNAP-8）是一种抗衰老肽，比乙酰六肽-3更有效，比肉毒杆菌毒素更稳定，有效缓解面部皱纹。含有SNAP-8的产品如贴片正在生产，但尚未报道分析方法测定SNAP-8。方法优化流动相、碰撞能量和脱溶剂线温度，采用液相色谱-三重四极杆质谱仪在多反应监测模式下新建SNAP-8质谱数据集。结果 开发的方法在最佳条件下显示出良好的线性 (r ≥ 0.9971)，定量限为 0.0125 ng/mL、重复性（% 相对标准偏差 = 0.02 至 0.12）和准确度（% 相对误差 = − 1.68 至 1.44）。该方法已成功应用于加载了 SNAP-8 的可生物降解微针贴片。结论 目前的 SNAP-8 定量方法可用于化妆品领域的质量控制。