The treatment inadequacy and toxicity associated with conventional anti-malarial, anti-inflammatory and analgesic drugs has called for the search of alternatives from medicinal plants, particularly, their phytochemicals with inherent pharmacological properties. In the present study, purified fraction of M. senegalensis leaf was evaluated for antimalarial, anti-inflammatory and analgesic properties. Antimalarial study was conducted against Plasmodium chabaudi and Plasmodium berghei using 4 days suppressive test, while anti-inflammatory and analgesic studies were conducted using egg albumin induced paw oedema and acetic acid induced pain model respectively. Sub-acute toxicity was assessed using serum biochemical parameters following 3 weeks administrations of the purified fraction. The purified fraction of M. senegalensis leaf shows dose dependent antiplasmodial activity with percentage curative effects of 15.24 ± 0.89, 45.70 ± 3.43 and 48.50 ± 4.56 at 75, 150 and 300 mg/kg bw against Plasmodium chabaudi and % curative effects of 44.25 ± 3.21, 72.74 ± 6.54 and 76.30 ± 8.32 respectively against Plasmodium berghei. The purified fraction exhibited 53.16 ± 4.09 and 60.76 ± 7.54 anti-inflammatory effect, 43.35 ± 4.98% and 44.83 ± 3.86% analgesic effect at 75 and 150 mg/kg bw respectively. GC-MS analysis confirmed the presence of 20α)-3-hydroxy-2-oxo-24-nor-friedela-1(10),3,5,7-tetraen-carboxylic acid-(29)-methylester, 2(4H)-Benzofuranone, 5,6,7,7a-tetrahydro- and 3-hydroxy-20(29)-lupen-28-ol and a terpenes (phytol) as the major antimalarial compounds in the fraction. The purified fraction increases the serum total proteins and transaminases concentrations but had no effect on serum levels of sodium, potassium, chloride, alkaline phosphatase, triglyceride and glucose in the mice. The purified fraction of M. senegalensis leaf exhibited promising antimalarial, analgesic and anti-inflammatory activities. Thus, could serve as a template for the synthesis of new drug.

中文翻译：

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塞内加尔Maytenus（lam。）Exell叶提取物在小鼠中的抗疟原虫，镇痛和抗炎成分的表征

与常规抗疟疾，抗炎和止痛药有关的治疗不足和毒性要求从药用植物中寻找替代物，特别是具有固有药理特性的植物化学物。在本研究中，评估了塞内加尔分支杆菌叶片的纯化级分的抗疟，抗炎和镇痛特性。使用4天抑制试验对chabaudi疟原虫和berghei疟原虫进行了抗疟研究，而使用蛋清诱导的爪水肿和乙酸诱导的疼痛模型分别进行了抗炎和镇痛研究。给予纯化级分3周后，使用血清生化参数评估亚急性毒性。M的纯化部分。塞内加尔的叶子显示出剂量依赖性抗疟原虫活性，在75、150和300 mg / kg bw时对沙巴氏疟原虫的治愈百分率分别为15.24±0.89、45.70±3.43和48.50±4.56，百分治愈率分别为44.25±3.21、72.74±6.54和76.30对伯氏疟原虫分别为±8.32。纯化的级分分别在75和150 mg / kg bw时显示出53.16±4.09和60.76±7.54的抗炎作用，43.35±4.98％和44.83±3.86％的镇痛作用。GC-MS分析确认存在20α）-3-羟基-2-氧代-24-nor-friedela-1（10），3,5,7-丁烯羧酸-（29）-甲酯，2（4H ）-苯并呋喃酮，5,6,7,7a-四氢和3-羟基20（29）-戊烯-28-醇和萜烯（植物醇）是该组分中的主要抗疟化合物。纯化的级分增加了血清总蛋白和转氨酶的浓度，但对小鼠血清钠，钾，氯化物，碱性磷酸酶，甘油三酸酯和葡萄糖水平没有影响。塞内加尔沙棘叶的纯化部分显示出有希望的抗疟，镇痛和抗炎活性。因此，可以作为合成新药的模板。