Herein, a simple, miniaturized and low-cost electrochemical sensing platform integrated with three-electrode system has been presented. A two-layer microfluidic device has been fabricated entirely on a PMMA sheet using a CO2 laser. Pencil graphite electrode (PGE), chemically modified electrode with a nanocomposite of MWCNT@polysterene-chitosan (PGE/MWCNT@PS-CS) as a working electrode (WE), PGE coated with Ag/AgCl ink as a reference electrode (RE) and a plain PGE as a counter electrode (CE) were inserted into the microreactor with reagent consumption of 2 mL. As a proof of concept, the analytical performance of the developed microfluidic electrochemical sensor was investigated for non-enzymatic glucose sensing. Highly selective, non-interfering sensing of glucose in presence of various bioanalytes, was obtained. The nanocomposite lead to an exceptional limit of detection as 0.309 nM. With fine-tuning and electrode surface modification, the designed miniaturized platform could be used for diverse electrochemical biosensing application.

中文翻译：

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具有纳米复合材料优化铅笔电极的小型化平台，用于选择性非干扰电化学传感

在此，提出了一种集成了三电极系统的简单、小型化和低成本的电化学传感平台。使用 CO2 激光器在 PMMA 片上完全制造了一个两层微流体装置。铅笔石墨电极 (PGE)，化学修饰电极，以 MWCNT@polysterene-chitosan (PGE/MWCNT@PS-CS) 的纳米复合材料作为工作电极 (WE)，涂有 Ag/AgCl 墨水的 PGE 作为参比电极 (RE)将普通 PGE 作为对电极 (CE) 插入微反应器中，试剂消耗量为 2 mL。作为概念证明，研究了开发的微流体电化学传感器的非酶葡萄糖传感分析性能。获得了在存在各种生物分析物的情况下对葡萄糖的高选择性、无干扰感测。纳米复合材料导致异常的检测限为 0.309 nM。通过微调和电极表面改性，设计的小型化平台可用于多种电化学生物传感应用。