Background: Diabetes Mellitus (DM) is a major public metabolic disease that influences 366 million people in the world in 2011, and this number is predicted to rise to 552 million in 2030. DM is clinically diagnosed by a fasting blood glucose that is equal or greater than 7 mM. Therefore, the development of effective glucose biosensor has attracted extensive attention worldwide. Fluorescence- based strategies have sparked tremendous interest due to their rapid response, facile operation, and excellent sensitivity. Many fluorescent compounds have been employed for precise analysis of glucose, including quantum dots, noble metal nanoclusters, up-converting nanoparticles, organic dyes, and composite fluorescent microspheres. Silicon dot as promising quantum dots materials have received extensive attention, owing to their distinct advantages such as biocompatibility, low toxicity and high photostability.

Methods: MnO2 nanosheets on the Si nanoparticles (NPs) surface serve as a quencher. Si NPs fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn2+, and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Therefore, the glucose concentration can be derived by recording the fluorescence recovery spectra of the Si NPs.

Results: This probe enabled selective detection of glucose with a linear range of 1-100 μg/mL and a limit of detection of 0.98 μg/mL. Compared with the commercial glucometer, this method showed favorable results and convincing reliability.

Conclusion: We have developed a novel method based on MnO2 -nanosheet-modified Si NPs for rapid monitoring of blood glucose levels. By combining the highly sensitive H2O2/MnO2 reaction with the excellent photostability of Si NPs, a highly sensitive, selective, and cost-efficient sensing approach for glucose detection has been designed and applied to monitor glucose levels in human serum with satisfactory results.

背景：糖尿病（DM）是一种主要的公共代谢疾病，2011年影响全球3.66亿人，预计这一数字将在2030年上升至5.52亿。在临床上，糖尿病是由空腹血糖等于或等于50的人诊断出来的大于7 mM。因此，有效的葡萄糖生物传感器的开发引起了全世界的广泛关注。基于荧光的策略因其快速响应，操作简便和出色的灵敏度而引起了极大的兴趣。许多荧光化合物已用于葡萄糖的精确分析，包括量子点，贵金属纳米簇，上转换纳米颗粒，有机染料和复合荧光微球。硅点作为有希望的量子点材料已受到广泛关注，

方法：Si纳米颗粒（NPs）表面上的MnO2纳米片用作淬灭剂。Si NPs荧光可以通过添加H2O2来恢复，这可以将MnO2还原为Mn2 +，因此可以基于葡萄糖通过葡萄糖氧化酶的酶促转化生成H2O2来监测葡萄糖。因此，可以通过记录Si NP的荧光恢复光谱来导出葡萄糖浓度。

结果：该探针能够选择性检测线性范围为1-100μg/ mL的葡萄糖，检测极限为0.98μg/ mL。与市售血糖仪相比，该方法具有良好的效果和令人信服的可靠性。

结论：我们已经开发了一种基于MnO2-纳米片修饰的Si NPs的新方法，用于快速监测血糖水平。通过将高灵敏度的H2O2 / MnO2反应与极高的Si NPs的光稳定性相结合，设计了一种高灵敏度，选择性高且经济高效的葡萄糖检测传感方法，并将其应用于监测人血清中的葡萄糖水平，结果令人满意。